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Sci. Pharm. 2014, 82(2), 375-392; doi:10.3797/scipharm.1310-25

Water Soluble Components of 'Osteocare' Promote Cell Proliferation, Differentiation, and Matrix Mineralization in Human Osteoblast-Like SaOS-2 Cells

1
Department of Cell Biology, Research and Development, The Himalaya Drug Company, Bangalore562 162, India
2
Department of Phytochemistry, Research and Development, The Himalaya Drug Company, Bangalore562 162, India
3
Medical Services and Clinical Trials, Research and Development, The Himalaya Drug Company, Bangalore-562 162, India
*
Author to whom correspondence should be addressed.
Received: 29 October 2013 / Accepted: 8 February 2014 / Published: 8 February 2014
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Abstract

Osteocare, a herbal formulation, has been found to be very effective in bone mineralization and support of the microstructure of bone tissue. The water-soluble components of Osteocare (WSCO) induced osteogenic activity in human osteoblast-like SaOS-2 cells. The addition of WSCO (100 μg/ml) to SaOS-2 cells was effective in increasing the cell proliferation by 41.49% and DNA content by 1.9-fold. WSCO increased matrix mineralization in SaOS-2 cells by increased alkaline phosphatase levels and calcium-rich deposits as observed by Alizarin red staining. WSCO markedly increased mRNA expression for osteopontin (OPN), osteocalcin (OCN), type I collagen (Col I) in SaOS-2 cells, and it down-regulated IL-6 mRNA levels in SaOS-2 cells. The present study showed that WSCO plays an important role in osteoblastic bone formation through enhanced activities of ALP, Col I, bone matrix proteins such as OPN and OCN, down-regulation of cytokines like IL-6, as well as promoting mineralization in SaOS-2 cells.
Keywords: Osteocare; Cell proliferation; ALP activity; OCN; OPN; Col-I; IL-6; Mineralization Osteocare; Cell proliferation; ALP activity; OCN; OPN; Col-I; IL-6; Mineralization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

VARMA, S.R.; SHARATH KUMAR, L.M.; VIDYASHANKAR, S.; PATKI, P.S. Water Soluble Components of 'Osteocare' Promote Cell Proliferation, Differentiation, and Matrix Mineralization in Human Osteoblast-Like SaOS-2 Cells. Sci. Pharm. 2014, 82, 375-392.

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