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Sci. Pharm. 2010, 78(4), 821-834; doi:10.3797/scipharm.1004-14

Simultaneous Quantitative Determination of Metoprolol, Atorvastatin and Ramipril in Capsules by a Validated Stability-Indicating RP-UPLC Method

1
Analytical Research and Development, Integrated Product Development, Dr. Reddy’s Laboratories Ltd., Bachupally, Hyderabad-500 072, India
2
Department of Chemistry, Rayalaseema University, Kurnool-518002, A.P., India
*
Author to whom correspondence should be addressed.
Received: 18 April 2010 / Accepted: 24 August 2010 / Published: 24 August 2010
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Abstract

A simple ultra performance liquid chromatographic (UPLC) method has been developed for the simultaneous estimation of Metoprolol (MT), Atorvastatin (AT) and Ramipril (RM) from capsule dosage form. The method was developed using Zorbax® XDB-C18 (4.6 mm x 50 mm, 1.8 μm) column with a mobile phase consisting of 0.06% ortho phosphoric acid in Milli Q® water having an ion pair reagent, 0.0045 M Sodium lauryl sulphate as buffer, at ratio of buffer: Acetonitrile (50:50 v/v), at 55°C column temperature with a flow rate of 1.0 ml/min. Detection was carried out with ultra-violet detection at 210 nm for RM, MT and AT respectively. The retention times were about 1.3, 2.1 and 2.6 min for MT, AT and RM respectively, the method was validated for linearity, accuracy, precision, specificity, robustness and ruggedness. The % mean recoveries are 101.9, 102.1 and 101.4 for MT, AT and RM respectively. The method was found to be rugged and robust and can be successfully used to determine the three drugs and its combinations.
Keywords: Fixed-dose combination; Chromatography; Method Validation; Reviro; Impurities Fixed-dose combination; Chromatography; Method Validation; Reviro; Impurities
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

SESHADRI, R.K.; DESAI, M.M.; RAGHAVARAJU, T.V.; KRISHNAN, D.; RAO, D.V.; CHAKRAVARTHY, I.E. Simultaneous Quantitative Determination of Metoprolol, Atorvastatin and Ramipril in Capsules by a Validated Stability-Indicating RP-UPLC Method. Sci. Pharm. 2010, 78, 821-834.

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