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Sci. Pharm. 2009, 77(3), 567-578; doi:10.3797/scipharm.0907-04

Preparation and Biological Evaluation of a [55Co]-2-Acetylpyridine Thiosemicarbazone

Agricultural, Medical and Industrial Research School (AMIRS), Nuclear Science and Technology Research Institute (NSTRI) Karaj, P.O.Box: 31485-498, Iran
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Received: 4 July 2009 / Accepted: 3 August 2009 / Published: 5 August 2009
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Abstract

Due to the anti-proliferative properties of cobalt-thiosemicarbazone complexes, the production of [55Co](III)-bis-(2-acetylpyridine thiosemicarbazone) ([55Co](III)[APTS]2) was investigated. Co-55 (T1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target by 15 MeV protons. The 55Co was separated from the irradiated target material using a two-step method with a radiochemical yield of >95% followed by radionuclidic and chemical purity control. [55Co](III)chloride was mixed with 2-acetylpyridine thiosemicarbazone for 30 min at room temperature to yield [55Co](III)[APTS]2 (radiochemical purity > 98% shown by RTLC/HPLC). A specific activity of about 10–20 Ci/mmol was obtained. The final solution was diluted in normal saline to 5% ethanolic solution for biological evaluation. The stability of the final product was checked in the absence and presence of human serum at 37°C to 24 h. The partition co-efficient of the final complex at the pH of 7 was 1.00±0.08. A significant tumor accumulation (%ID/g; 3.5%) was observed in tumoral tissue 21 h post injection in fibrosarcoma-bearing mice by biodistribution studies. Co-incidence imaging also demonstrated tumor uptake from 21–35 h however at 35 h tumor uptake is more specific and significant.
Keywords: Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

JALILIAN, A.R.; ROWSHANFARZAD, P.; AKHLAGHI, M.; SABET, M.; KAMALI-DEHGHAN, M.; POULADI, M. Preparation and Biological Evaluation of a [55Co]-2-Acetylpyridine Thiosemicarbazone. Sci. Pharm. 2009, 77, 567-578.

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