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Sci. Pharm. 2006, 74(3), 137-149; doi:10.3797/scipharm.2006.74.137 (registering DOI)

Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying

Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria
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Author to whom correspondence should be addressed.
Received: 2 July 2006 / Accepted: 3 September 2006 / Published: 30 September 2006
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Abstract

The effect of different protectants and the impact of the initial cell density on the viability of Lactococcus lactis Sr. 3.54 subjected to freeze-thawing and freezedrying was studied. Several additives were tested as protective agents against freezing and drying injuries. Maximum viability of the cells after freeze-thawing was obtained with sucrose and skim milk mixtures as protective agents (78% viability). Freeze-drying with protectants based on skim milk or MRS-broth were most effective (survival levels >60%). The effect of the initial cell load on the final recovery was dependent on the protectant. Every sample showed an increase in viability when a high initial cell concentration (1010cfu ml-1) was used. The blank showed a 1500 fold increase, skim milk/sucrose based lyophilisates an 1,7 fold increase in viability when the initial cell load was changed from 1009 to 1010cfu ml-1. The use of 1010cfu ml-1 as initial cell concentration and sucrose/skim milk as protectant yielded a lyophilisate with 71% viability. Results suggest the possibility of producing freeze dried powders of Lactococcus lactis with high viability for the food industry.
Keywords: Lactococcus lactis; freeze-drying; freeze-thawing; lactic acid bacteria; initial cell concentration Lactococcus lactis; freeze-drying; freeze-thawing; lactic acid bacteria; initial cell concentration
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Berner, D.; Viernstein, H. Effect of protective agents on the viability of Lactococcus lactis subjected to freeze-thawing and freeze-drying. Sci. Pharm. 2006, 74, 137-149.

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