Design and Characterization of a Minimally Invasive Bipolar Electrode for Electroporation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Theoretical Study Results
2.1.1. Symmetric Geometry
2.1.2. Asymmetric Geometry
2.2. Vegetable Model Results
2.3. Animal Model Results
3. Discussions and Conclusions
4. Materials and Methods
4.1. Electrode Configuration
4.2. Comsol Multiphysics®
4.3. Theoretical Study
4.4. Prototype Design
4.5. Vegetable Model
4.6. Animal Model
4.7. Histological Analysis
4.8. Immunohistochemical Analysis
Author Contributions
Funding
Conflicts of Interest
References
- Impellizeri, J.; Aurisicchio, L.; Forde, P.; Soden, D.M. Electroporation in veterinary oncology. Vet. J. 2016, 217, 18–25. [Google Scholar] [CrossRef]
- Lee, E.W.; Gehl, J.; Kee, S.T. Introduction to Electroporation. In Clinical Aspects of Electroporation; Kee, S., Gehl, J., Lee, E., Eds.; Springer: New York, NY, USA, 2011; pp. 3–7. [Google Scholar] [CrossRef]
- Cadossi, R.; Ronchetti, M.; Cadossi, M. Locally enhanced chemotherapy by electroporation: Clinical experiences and perspective of use of electrochemotherapy. Futur. Oncol. 2014, 10, 877–890. [Google Scholar] [CrossRef] [Green Version]
- Ongaro, A.; Pellati, A.; Caruso, A.; Battista, M.; De Terlizzi, F.; De Mattei, M.; Fini, M. Identification of in vitro electropermeabilization equivalent pulse protocols. Technol. Cancer Res. Treat. 2011, 10, 465–473. [Google Scholar] [CrossRef]
- Zhao, Y.; Liu, H.; Bhonsle, S.P.; Wang, Y.; Davalos, R.V.; Yao, C. Ablation outcome of irreversible electroporation on potato monitored by impedance spectrum under multi-electrode system. Biomed. Eng. Online 2018, 17, 1–13. [Google Scholar] [CrossRef] [Green Version]
- Davalos, R.V.; Mir, L.M.; Rubinsky, B. Tissue ablation with irreversible electroporation. Ann. Biomed. Eng. 2005, 33, 223–231. [Google Scholar] [CrossRef] [PubMed]
- Rubinsky, B. Irreversible electroporation in medicine. Technol Cancer Res. Treat. 2007, 6, 255–260. [Google Scholar] [CrossRef] [PubMed]
- Thomson, K.R.; Cheung, W.; Ellis, S.J.; Park, D.; Kavnoudias, H.; Loader-Oliver, D.; Roberts, S.; Evans, P.; Ball, C.; Haydon, A. Investigation of the Safety of Irreversible Electroporation in Humans. J. Vasc. Interv. Radiol. 2011, 22, 611–621. [Google Scholar] [CrossRef] [PubMed]
- Edd, J.F.; Horowitz, L.; Davalos, R.V.; Mir, L.M.; Rubinsky, B. In vivo results of a new focal tissue ablation technique:irreversible electroporation. IEEE Trans, Biomed. Eng. 2006, 53, 1409–1415. [Google Scholar] [CrossRef]
- Maor, E.; Ivorra, A.; Leor, J.; Rubinsky, B. The effect of irreversible electroporation on blood vessels. Technol. Cancer Res. 2007, 6, 307–312. [Google Scholar] [CrossRef] [PubMed]
- Maor, E.; Ivorra, A.; Rubinsky, B. Non Thermal Irreversible Electroporation: Novel Technology for Vascular Smooth Muscle Cells Ablation. PLoS ONE 2009, 4, e4757. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Orlowski, S.; Belehradek, J., Jr.; Paoletti, C.; Mir, L.M. Transient electropermeabilization of cells in culture. Increase of the cytotoxicity of anticancer drugs. Biochem. Pharmacol. 1988, 37, 4727–4733. [Google Scholar] [CrossRef]
- Sersa, G.; Gehl, J.; Garbay, J.R.; Soden, D.M.; O’Sullivan, G.C.; Matthiessen, L.W.; Snoj, M.; Mir, L.M. Electrochemotherapy of Small Tumors; The Experience from the ESOPE (European Standard Operating Procedures for Electrochemotherapy) Group. In Clinical Aspects of Electroporation; Kee, S., Gehl, J., Lee, E., Eds.; Springer: New York, NY, USA, 2011; Chapter 8; pp. 93–102. Available online: https://doi.org/10.1007/978-1-4419-8363-3_8 (accessed on 18 November 2019).
- “Cliniporator”. Available online: https://www.igea.it/it/oncologia/area-dedicata-al-medico (accessed on 18 November 2019).
- Miklavčič, D. Network for development of electroporation-based technologies and treatments. J. Membr. Biol. 2012, 245, 591–598. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Yarmush, M.; Golberg, L.; Serša, A.; Kotnik, G.T.; Miklavčič, D. Electroporation-based technologies for medicine: Principles, applications, and challenge. Annu. Rev. Biomed. Eng. 2014, 16, 295–320. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kotnik, T.; Frey, W.; Sack, M.; Meglič, S.H.; Peterka, M.; Miklavčič, D. Electroporation-based applications in biotechnology. Trends Biotechnol. 2015, 33, 480–488. [Google Scholar] [CrossRef] [PubMed]
- Mahniˇc-Kalamiza, S.; Vorobiev, E.; Miklavčič, D. Electroporation in food processing and biorefinery. J. Membr. Biol. 2014, 247, 1279–1304. [Google Scholar] [CrossRef]
- Campana, L.G.; Miklavčič, D.; Bertino, G.; Marconato, R.; Valpione, S.; Imarisio, I.; Dieci, M.V.; Granziera, E.; Cˇemazˇar, M.; Alaibac, M.; et al. Electrochemotherapy of superficial tumors – current status: Basic principles, operating procedures, shared indications, and emerging applications. Semin. Oncol. 2019, 46, 173–191. [Google Scholar] [CrossRef] [Green Version]
- Girelli, R.; Frigerio, I.; Salvia, R.; Barbi, E.; Tinazzi Martini, P.; Bassi, C. Feasibility and safety of radiofrequency ablation for locally advanced pancreatic cancer. Br. J. Surg. 2010, 97, 220–225. [Google Scholar] [CrossRef]
- Miklavčič, D.; Serša, G.; Brecelj, E.; Gehl, J.; Soden, D.; Bianchi, G.; Ruggieri, P.; Rossi, C.R.; Campana, L.G.; Jarme, T. Electrochemotherapy: Technological advancements for efficient electroporation-based treatment of internal tumors. Med. Biol. Eng. Comput. 2012, 50, 1213–1225. [Google Scholar] [CrossRef] [Green Version]
- Mavrogenis, A.F.; Angelini, A.; Vottis, C.; Pala, E.; Calabrò, T.; Papagelopoulos, P.; Ruggieri, P. Modern palliative treatments for metastatic bone disease: Awareness of merits, demerits and guidance. Clin. J. Pain. 2016, 32, 337–350. [Google Scholar] [CrossRef]
- Fini, M.; Salamanna, F.; Parrilli, A.; Martini, L.; Cadossi, M.; Maglio, M.; Borsari, V. Electrochemotherapy is effective in the treatment of rat bone metastases. Clin. Exp. Metastasis. 2013, 30, 1033–1045. [Google Scholar] [CrossRef]
- Edhemovic, I.; Gadzijev, E.M.; Brecelj, E.; Miklavcic, D.; Kos, B.; Zupanic, A.; Mali, B.; Jarm, T.; Pavliha, D.; Marcan, M.; et al. Electrochemotherapy: A new technological approach in treatment of metastases in the liver. Technol. Cancer Res. Treat. 2011, 10, 475–485. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fini, M.; Tschon, M.; Ronchetti, M.; Cavani, F.; Bianchi, G.; Mercuri, M.; Alberghini, M.; Cadossi, R.J. Ablation of bone cells by electroporation. Bone Joint. Surg. Br. 2010, 1614–1620. [Google Scholar] [CrossRef] [PubMed]
- Gasbarrini, A.; Campos, W.; Campanacci, L.; Boriani, S. Electrochemotherapy to Metastatic Spinal Melanoma. Spine 2015, 40, E1340–E1346. [Google Scholar] [CrossRef]
- Bianchi, G.; Campanacci, L.; Ronchetti, M.; Donati, D. Electrochemotherapy in the Treatment of Bone Metastases: A Phase II Trial. World J. Surg. 2016, 40, 3088–3094. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Cornelis, F.H.; Ammar, M.B.; Nouri-Neville, M.; Matton, L.; Benderra, M.A.; Glogorov, J.; Fallet, V.; Mir, L.M. Percutaneous Image-Guided Electrochemotherapy of Spine Metastases: Initial Experience. Cardiovasc. Intervent. Radiol. 2019, 42, 1806–1809. [Google Scholar] [CrossRef] [PubMed]
- Tarantino, L.; Busto, G.; Nasto, A.; Fristachi, R.; Cacace, L.; Talamo, M.; Accardo, C.; Bortone, S.; Gallo, P.; Tarantino, P.; et al. Percutaneous electrochemotherapy in the reatment of portal vein tumor thrombosis at hepatic hilum in patients with hepatocellular carcinoma in cirrhosis: A feasibility study. World J. Gastroenterol. 2017, 23, 906–918. [Google Scholar] [CrossRef]
- Tarantino, L.; Busto, G.; Nasto, A.; Nasto, R.A.; Tarantino, P.; Fristachi, R.; Cacace, L.; Bortone, S. Ectrochemotherapy of cholangiocellular carcinoma at hepatic hilum: A feasibility study. Eur. J. Surg. Oncol. 2018, 44, 1603–1609. [Google Scholar] [CrossRef]
- Granata, V.; Fusco, R.; Setola, S.V.; Piccirillo, M.; Leongito, M.; Palaia, R.; Granata, F.; Lastoria, S.; Izzo, F.; Petrillo, A. Early radiological assessment of locally advanced pancreatic cancer treated with electrochemotherapy. World J. Gastroenterol. 2017, 23, 4767–4778. [Google Scholar] [CrossRef]
- Cornelis, F.; Korenbaum, C.; Ben Ammar, M.; Tavolaro, S.; Nouri-Neuville, M.; Lotz, J.P. Multimodal image-guided electrochemotherapy of unresectable liver metastasis from renal cell cancer. Diagn. Interv. Imaging. 2019, 100, 309–311. [Google Scholar] [CrossRef]
- Izzo, F.; Ionna, F.; Granata, V.; Albino, V.; Patrone, R.; Longo, F.; Guida, A.; Delrio, P.; Rega, D.; Scala, D.; et al. New Deployable Expandable Electrodes in the Electroporation Treatment in a Pig Model: A Feasibility and Usability Preliminary Study. Cancers 2020, 12, 515. [Google Scholar] [CrossRef] [Green Version]
- “Comsol Multiphysics”. Available online: https://www.comsol.com/ (accessed on 14 November 2019).
- Wandel, A.; Ben-David, E.; Ulusoy, B.S.; Neal, R.; Faruja, M.; Nissenbaum, I.; Gourovich, S.; Goldberg, S.N. Optimizing Irreversible Electroporation Ablation with a Bipolar Electrode. J. Vasc. Interv. Radiol. 2016, 27, 1441–1450. [Google Scholar] [CrossRef] [PubMed]
- Edhemovic, I.; Brecelj, E.; Gasljevic, G.; Music, M.M.; Gorjup, V.; Mali, B.T.; Jarm, B.K.; Pavliha, D.; Kuzmanov, B.G.; Cemazar, M.; et al. Intraoperative electrochemotherapy of colorectal liver metastases. J. Surg. Oncol. 2014, 110, 320–327. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Neal, R.E.; Singh, R.; Hatcher, H.C.; Kock, N.D.; Torti, S.V.; Davalos, R.V. Treatment of breast cancer through the application of IRE using a novel minimally invasive single needle electrode. Breast Cancer Res. Treat. 2010, 123, 295–301. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Neal, R.E.; Kavnoudias, H.; Thomson, K.R. An “off-the-shelf” system for intraprocedural electrical current evaluation and monitoring of irreversible electroporation therapy. Cardiovasc. Intervent. Radiol. 2015, 38, 736–741. [Google Scholar] [CrossRef]
- Grimnes, S.; Martinsen, Ø.G. (Eds.) Bioimpedance and Bioelectricity Basics, 2nd ed.; Academic Press: London, UK, 2008. [Google Scholar]
- Auriemma, F.; De Luca, L.; Bianchetti, M.; Repici, A.; Mangiavillano, B. Radiofrequency and malignant biliary strictures: An update. World. J. Gastrointest. Endosc. 2019, 11, 95–102. [Google Scholar] [CrossRef]
- Buerlein, R.C.D.; Wang, A.Y. Endoscopic Retrograde Cholangiopancreatography-Guided Ablation for Cholangiocarcinoma. Gastrointest. Endosc. Clin. 2019, 29, 351–367. [Google Scholar] [CrossRef]
- Patel, J.; Rizk, N.; Kahaleh, M. Role of photodynamic therapy and intraductal radiofrequency ablation in cholangiocarcinoma. Best Pract. Res. Clin. Gastroenterol. 2015, 29, 309–318. [Google Scholar] [CrossRef]
- Zheng, X.; Bo, Z.Y.; Wan, W.; Wu, Y.C.; Wang, T.T.; Wu, J.; Gao, D.J.; Hu, B. Endoscopic radiofrequency ablation may be preferable in the management of malignant biliary obstruction: A systematic review and meta-analysis. J. Dig. Dis. 2016, 17716–17724. [Google Scholar] [CrossRef]
- Simon, C.J.; Dupuy, D.E.; Mayo-Smith, W.W. Microwave ablation: Principles and applications. Radiographics 2005, 1, S69–S83. [Google Scholar] [CrossRef]
- Fallahi, H.; Prakash, P. Antenna Designs for Microwave Tissue Ablation. Crit. Rev. Biomed. Eng. 2018, 46, 495–521. [Google Scholar] [CrossRef]
- Eshet, Y.; Mann, R.R.; Anaton, A.; Yacoby, T.; Gefen, A.; Jerby, E. Microwave drilling of bones. IEEE Trans. Biomed. Eng. 2006, 53, 1174–1182. [Google Scholar] [CrossRef] [PubMed]
- Colebeck, E.; Topsakal, E. Ultra-wideband Microwave Ablation Therapy (UMAT). In IEEE MTT-S International Microwave Symposium Digest; IEEE: Seattle, WA, USA, 2013; pp. 1–3. [Google Scholar] [CrossRef]
- Kaushal, V.; Herzog, C.; Haun, R.S.; Kaushal, G.P. Caspase protocols in mice. Methods Mol. Biol. 2014, 1133, 141–154. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Test Session | Changed Variable | Conductive Pole P1 (mm) | Insulated Pole S (mm) | Conductive Pole P2 (mm) | Voltage (V) | Diameter D (mm) | Diameter of Electroporated Volume along P1 (mm) | Diameter of Electroporated Volume along S (mm) | Diameter of Electroporated Volume along P2 (mm) |
---|---|---|---|---|---|---|---|---|---|
1 | Diameter | 5.00 | 3.00 | 5.00 | 1000 | 1.40 | 10.00 | 9.00 | 10.00 |
5.00 | 3.00 | 5.00 | 1000 | 1.50 | 10.00 | 9.00 | 10.00 | ||
5.00 | 3.00 | 5.00 | 1000 | 1.60 | 10.50 | 9.00 | 10.50 | ||
5.00 | 3.00 | 5.00 | 1000 | 1.80 | 11.00 | 10.00 | 11.00 | ||
5.00 | 3.00 | 5.00 | 1000 | 2.00 | 11.00 | 10.00 | 11.00 | ||
2 | Voltage | 5.00 | 3.00 | 5.00 | 500 | 1.40 | 8.00 | 7.00 | 8.00 |
5.00 | 3.00 | 5.00 | 1000 | 1.40 | 10.00 | 9.00 | 10.00 | ||
5.00 | 3.00 | 5.00 | 1500 | 1.40 | 14.00 | 13.00 | 14.00 | ||
3 | Spacer | 5.00 | 3.00 | 5.00 | 1000 | 1.40 | 10.00 | 9.00 | 10.00 |
5.00 | 5.00 | 5.00 | 1000 | 1.40 | 10.00 | 8.00 | 10.00 | ||
5.00 | 7.00 | 5.00 | 1000 | 1.40 | 10.00 | 7.00 | 10.00 | ||
4 | Conductive poles | 3.00 | 3.00 | 3.00 | 1000 | 1.40 | 9.00 | 9.00 | 9.00 |
5.00 | 3.00 | 5.00 | 1000 | 1.40 | 10.00 | 9.00 | 10.00 | ||
10.00 | 3.00 | 10.00 | 1000 | 1.40 | 12.00 | 11.00 | 12.00 | ||
15.00 | 3.00 | 15.00 | 1000 | 1.40 | 14.00 | 13.00 | 14.00 | ||
5 | Voltage | 5.00 | 3.00 | 10.00 | 500 | 1.40 | 12.00 | 10.00 | 8.00 |
5.00 | 3.00 | 10.00 | 800 | 1.40 | 14.00 | 12.00 | 10.00 | ||
5.00 | 3.00 | 10.00 | 1000 | 1.40 | 16.00 | 14.00 | 12.00 | ||
5.00 | 3.00 | 10.00 | 1500 | 1.40 | 18.00 | 16.00 | 14.00 | ||
6 | Voltage and Diameter | 5.00 | 3.00 | 10.00 | 500 | 1.40 | 12.00 | 10.00 | 8.00 |
5.00 | 3.00 | 10.00 | 500 | 1.50 | 12.00 | 10.00 | 8.00 | ||
5.00 | 3.00 | 10.00 | 800 | 1.40 | 14.00 | 12.00 | 10.00 | ||
5.00 | 3.00 | 10.00 | 800 | 1.50 | 14.00 | 12.00 | 10.00 | ||
5.00 | 3.00 | 10.00 | 1000 | 1.40 | 16.00 | 14.00 | 12.00 | ||
5.00 | 3.00 | 10.00 | 1000 | 1.50 | 16.00 | 14.00 | 12.00 | ||
5.00 | 3.00 | 10.00 | 1500 | 1.40 | 18.00 | 16.00 | 14.00 | ||
5.00 | 3.00 | 10.00 | 1500 | 1.50 | 18.00 | 16.00 | 14.00 | ||
7 | Spacer | 5.00 | 3.00 | 10.00 | 500 | 1.40 | 12.00 | 10.00 | 8.00 |
5.00 | 5.00 | 10.00 | 500 | 1.40 | 12.00 | 5.00 | 8.00 | ||
8 | Conductive Poles | 20.00 | 3.00 | 5.00 | 500 | 1.40 | 3.00 | 8.00 | 12.00 |
15.00 | 3.00 | 5.00 | 500 | 1.40 | 4.00 | 8.00 | 10.00 | ||
10.00 | 3.00 | 5.00 | 500 | 1.40 | 6.00 | 8.00 | 10.00 | ||
10.00 | 3.00 | 3.00 | 500 | 1.40 | 6.00 | 7.00 | 8.00 | ||
5.00 | 3.00 | 20.00 | 500 | 1.40 | 12.00 | 8.00 | 3.00 | ||
5.00 | 3.00 | 15.00 | 500 | 1.40 | 10.00 | 8.00 | 4.00 | ||
5.00 | 3.00 | 10.00 | 500 | 1.40 | 10.00 | 8.00 | 6.00 | ||
3.00 | 3.00 | 10.00 | 500 | 1.40 | 8.00 | 7.00 | 6.00 |
P1 (mm) | S (mm) | P2 (mm) | Total Length (mm) | Voltage (V) | Diameter of Electroporated Volume along P1 (mm) | Diameter of Electroporated Volume along S (mm) | Diameter of Electroporated Volume along P2 (mm) | |
---|---|---|---|---|---|---|---|---|
1 | 10.00 | 3.00 | 10.00 | 23.00 | 500 | 12.30 | 14.30 | 12.10 |
2 | 10.00 | 5.00 | 10.00 | 25.00 | 800 | 15.20 | 18.20 | 10.00 |
3 | 10.00 | 5.00 | 10.00 | 25.00 | 500 | 12.75 | 15.55 | 8.50 |
4 | 10.00 | 3.00 | 5.00 | 18.00 | 500 | 13.20 | 14.90 | 9.50 |
5 | 5.00 | 5.00 | 10.00 | 20.00 | 800 | 12.30 | 15.40 | 13.45 |
6 | 5.00 | 3.00 | 5.00 | 13.00 | 500 | 12.75 | 14.95 | 12.50 |
7 | 5.00 | 5.00 | 5.00 | 15.00 | 800 | 9.60 | 16.90 | 12.55 |
8 | 5.00 | 3.00 | 15.00 | 23.00 | 500 | 12.10 | 10.80 | 9.50 |
9 | 5.00 | 5.00 | 15.00 | 25.00 | 800 | 15.60 | 18.45 | 14.75 |
10 | 3.00 | 3.00 | 3.00 | 9.00 | 500 | 8.70 | 7.90 | 8.50 |
11 | 3.00 | 5.00 | 3.00 | 11.00 | 800 | 13.40 | 14.10 | 12.90 |
12 | 3.00 | 3.00 | 10.00 | 16.00 | 500 | 10.65 | 9.90 | 7.50 |
13 | 3.00 | 5.00 | 10.00 | 18.00 | 800 | 17.95 | 18.50 | 12.55 |
14 | 3.00 | 5.00 | 10.00 | 18.00 | 500 | 12.40 | 12.10 | 10.35 |
Test | Electrode | P1 (mm) | S (mm) | P2 (mm) | D (P1) (mm) | D (P2) (mm) | Voltage (V) | No. of Pulses | Parenchymal Damage (mm2) |
---|---|---|---|---|---|---|---|---|---|
1 | 1 | 5.00 | 3.00 | 20.00 | 1.80 | 2.00 | 800 | 80 | 9.18 × 10.03 |
2 | 1 | 5.00 | 3.00 | 20.00 | 1.80 | 2.00 | 1200 | 80 | 8.89 × 7.19 |
3 | 2 | 5.00 | 3.00 | 10.00 | 1.80 | 2.00 | 800 | 80 | 12.99 × 11.82 |
4 | 2 | 5.00 | 3.00 | 10.00 | 1.80 | 2.00 | 1200 | 80 | 8.06 × 9.56 |
AISI 4340 | Unit of Measure | |
---|---|---|
Density | 7850 | kg/m3 |
Young’s modulus | 205 × 109 | Pa |
Thermal conductivity | 44.5 | W/mK |
Specific heat | 475 | J/kgK |
Electric conductivity | 4.032 × 106 | S/m |
Thermal expansion coefficient | 12.3 × 10−6 | K−1 |
Relative permittivity | 1 | - |
Poisson’s ratio | 0.28 | - |
Properties | Polyimide | Unit of Measure |
---|---|---|
Density | 1300 | kg/m3 |
Young’s modulus | 3.1 × 109 | Pa |
Thermal conductivity | 0.15 | W/mK |
Specific heat | 1100 | J/kgK |
Electric conductivity | 10−10 | S/m |
Relative permittivity | 4 | - |
Poisson’s ratio | 0.28 | - |
Properties | Animal Liver | Unit of Measure |
---|---|---|
Electric conductivity | 0.07 | S/m |
Relative permittivity | 80 | - |
Outer Diameter (OD) | Inner Diameter (ID) | Wall Thickness | |
---|---|---|---|
Stainless-steel mandrel | 1.40 ± 0.02 mm | - | - |
Reduced stainless-steel mandrel | 0.83 ± 0.02 mm | - | - |
First insulated sheath | 0.9144 mm | 0.8636 ± 0.010 mm | 0.0254 ± 0.0064 mm |
Second conductive pole | 1.15 ± 0.02 mm | 0.95 ± 0.02 mm | 0.20 |
Second insulated sheath | 1.3843 mm | 1.1938 ± 0.013 mm | 0.0953 ± 0.0239 mm |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Merola, G.; Fusco, R.; Di Bernardo, E.; D’Alessio, V.; Izzo, F.; Granata, V.; Contartese, D.; Cadossi, M.; Audenino, A.; Perazzolo Gallo, G. Design and Characterization of a Minimally Invasive Bipolar Electrode for Electroporation. Biology 2020, 9, 303. https://doi.org/10.3390/biology9090303
Merola G, Fusco R, Di Bernardo E, D’Alessio V, Izzo F, Granata V, Contartese D, Cadossi M, Audenino A, Perazzolo Gallo G. Design and Characterization of a Minimally Invasive Bipolar Electrode for Electroporation. Biology. 2020; 9(9):303. https://doi.org/10.3390/biology9090303
Chicago/Turabian StyleMerola, Giulia, Roberta Fusco, Elio Di Bernardo, Valeria D’Alessio, Francesco Izzo, Vincenza Granata, Deyanira Contartese, Matteo Cadossi, Alberto Audenino, and Giacomo Perazzolo Gallo. 2020. "Design and Characterization of a Minimally Invasive Bipolar Electrode for Electroporation" Biology 9, no. 9: 303. https://doi.org/10.3390/biology9090303