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Biology 2016, 5(2), 22; doi:10.3390/biology5020022

Effect of Different Broad Waveband Lights on Membrane Lipids of a Cyanobacterium, Synechococcus sp., as Determined by UPLC-QToF-MS and Vibrational Spectroscopy

1
Centre for Biotechnology Development (CDB), Spanish Council for Scientific Research (CSIC), Francisco Vallés 8, Valladolid 47151, Spain
2
Fisica de la Materia Condensada Department, University of Valladolid, Valladolid 47011, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Annette Graham
Received: 28 March 2016 / Revised: 17 May 2016 / Accepted: 18 May 2016 / Published: 23 May 2016
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Abstract

Differential profile of membrane lipids and pigments of a Synechococcus sp. cyanobacterial strain cells exposed to blue, green, red and white light are determined by means of liquid chromatography and mass spectrometry or diode array detection. Raman and ATR-IR spectra of intact cells under the diverse light wavebands are also reported. Blue light cells exhibited an increased content of photosynthetic pigments as well as specific species of membrane glycerolipids as compared to cells exposed to other wavebands. The A630/A680 ratio indicated an increased content of phycobilisomes (PBS) in the blue light-exposed cells. Some differences in the protein conformation between the four light waveband-exposed cells were deduced from the variable absorbance at specific wavenumbers in the FT-Raman and ATR-FTIR spectra, in particular bands assigned to amide I and amide II. Bands from 1180 to 950 cm−1 in the ATR-FTIR spectrum suggest degraded outer membrane polysaccharide in the blue light-exposed cells. View Full-Text
Keywords: Synechococcus sp.; UPLC-MS; vibrational spectroscopy; Raman; IR; lipids; pigments; light quality Synechococcus sp.; UPLC-MS; vibrational spectroscopy; Raman; IR; lipids; pigments; light quality
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Montero, O.; Velasco, M.; Sanz-Arranz, A.; Rull, F. Effect of Different Broad Waveband Lights on Membrane Lipids of a Cyanobacterium, Synechococcus sp., as Determined by UPLC-QToF-MS and Vibrational Spectroscopy. Biology 2016, 5, 22.

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