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Mammalian Non-CpG Methylation: Stem Cells and Beyond
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Biology 2016, 5(1), 3; doi:10.3390/biology5010003

DNA Methylation Analysis: Choosing the Right Method

1
Genomics Core facility, Kolling Institute of Medical Research, University of Sydney, Sydney 2065, Australia
2
Cancer Genetics Laboratory, Kolling Institute of Medical Research, University of Sydney, Sydney 2065, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Melanie Ehrlich
Received: 8 July 2015 / Revised: 16 December 2015 / Accepted: 22 December 2015 / Published: 6 January 2016
(This article belongs to the Special Issue DNA Methylation)
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Abstract

In the burgeoning field of epigenetics, there are several methods available to determine the methylation status of DNA samples. However, choosing the method that is best suited to answering a particular biological question still proves to be a difficult task. This review aims to provide biologists, particularly those new to the field of epigenetics, with a simple algorithm to help guide them in the selection of the most appropriate assay to meet their research needs. First of all, we have separated all methods into two categories: those that are used for: (1) the discovery of unknown epigenetic changes; and (2) the assessment of DNA methylation within particular regulatory regions/genes of interest. The techniques are then scrutinized and ranked according to their robustness, high throughput capabilities and cost. This review includes the majority of methods available to date, but with a particular focus on commercially available kits or other simple and straightforward solutions that have proven to be useful. View Full-Text
Keywords: DNA methylation; 5-methylcytosine; CpG islands; epigenetics; next generation sequencing DNA methylation; 5-methylcytosine; CpG islands; epigenetics; next generation sequencing
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Kurdyukov, S.; Bullock, M. DNA Methylation Analysis: Choosing the Right Method. Biology 2016, 5, 3.

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