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Biology 2012, 1(2), 339-369; doi:10.3390/biology1020339
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Transcriptional Gene Silencing (TGS) via the RNAi Machinery in HIV-1 Infections

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Received: 29 June 2012; in revised form: 3 August 2012 / Accepted: 13 August 2012 / Published: 24 August 2012
(This article belongs to the Special Issue Structural and Molecular Biology of HIV)
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Abstract: Gene silencing via non-coding RNA, such as siRNA and miRNA, can occur at the transcriptional, post-transcriptional, and translational stages of expression. Transcriptional gene silencing (TGS) involving the RNAi machinery generally occurs through DNA methylation, as well as histone post-translational modifications, and corresponding remodeling of chromatin around the target gene into a heterochromatic state. The mechanism by which mammalian TGS occurs includes the recruitment of RNA-induced initiation of transcriptional gene silencing (RITS) complexes, DNA methyltransferases (DNMTs), and other chromatin remodelers. Additionally, virally infected cells encoding miRNAs have also been shown to manipulate the host cell RNAi machinery to induce TGS at the viral genome, thereby establishing latency. Furthermore, the introduction of exogenous siRNA and shRNA into infected cells that target integrated viral promoters can greatly suppress viral transcription via TGS. Here we examine the latest findings regarding mammalian TGS, specifically focusing on HIV-1 infected cells, and discuss future avenues of exploration in this field.
Keywords: HIV; miRNA; RNAi; transcriptional gene silencing; chromatin remodeler HIV; miRNA; RNAi; transcriptional gene silencing; chromatin remodeler
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sampey, G.C.; Guendel, I.; Das, R.; Jaworski, E.; Klase, Z.; Narayanan, A.; Kehn-Hall, K.; Kashanchi, F. Transcriptional Gene Silencing (TGS) via the RNAi Machinery in HIV-1 Infections. Biology 2012, 1, 339-369.

AMA Style

Sampey GC, Guendel I, Das R, Jaworski E, Klase Z, Narayanan A, Kehn-Hall K, Kashanchi F. Transcriptional Gene Silencing (TGS) via the RNAi Machinery in HIV-1 Infections. Biology. 2012; 1(2):339-369.

Chicago/Turabian Style

Sampey, Gavin C.; Guendel, Irene; Das, Ravi; Jaworski, Elizabeth; Klase, Zachary; Narayanan, Aarthi; Kehn-Hall, Kylene; Kashanchi, Fatah. 2012. "Transcriptional Gene Silencing (TGS) via the RNAi Machinery in HIV-1 Infections." Biology 1, no. 2: 339-369.


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