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Biosensors 2017, 7(2), 16; doi:10.3390/bios7020016

Electrochemical Field-Effect Transistor Utilization to Study the Coupling Success Rate of Photosynthetic Protein Complexes to Cytochrome c

1
Department of Electrical Engineering, University of South Florida (USF), Tampa, FL 33620, USA
2
Department of Chemistry, University of California Irvine, Irvine, CA 92697, USA
3
Department of Microbiology and Immunology, University of British Columbia (UBC), Vancouver, BC V6T1Z3, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Jeff D. Newman
Received: 19 February 2017 / Revised: 17 March 2017 / Accepted: 27 March 2017 / Published: 30 March 2017
(This article belongs to the Special Issue Biophotonic Sensors and Applications)
View Full-Text   |   Download PDF [1387 KB, uploaded 30 March 2017]   |  

Abstract

Due to the high internal quantum efficiency, reaction center (RC) proteins from photosynthetic organisms have been studied in various bio-photoelectrochemical devices for solar energy harvesting. In vivo, RC and cytochrome c (cyt c; a component of the biological electron transport chain) can form a cocomplex via interprotein docking. This mechanism can be used in vitro for efficient electron transfer from an electrode to the RC in a bio-photoelectrochemical device. Hence, the success rate in coupling RCs to cyt c is of great importance for practical applications in the future. In this work, we use an electrochemical transistor to study the binding of the RC to cytochrome. The shift in the transistor threshold voltage was measured in the dark and under illumination to estimate the density of cytochrome and coupled RCs on the gate of the transistor. The results show that ~33% of the cyt cs on the transistor gate were able to effectively couple with RCs. Due to the high sensitivity of the transistor, the approach can be used to make photosensors for detecting low light intensities. View Full-Text
Keywords: bio-photosensor; reaction center; rhodobacter sphaeroides; charge density; protein-protein interaction bio-photosensor; reaction center; rhodobacter sphaeroides; charge density; protein-protein interaction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Takshi, A.; Yaghoubi, H.; Wang, J.; Jun, D.; Beatty, J.T. Electrochemical Field-Effect Transistor Utilization to Study the Coupling Success Rate of Photosynthetic Protein Complexes to Cytochrome c. Biosensors 2017, 7, 16.

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