Single Molecule Bioelectronics and Their Application to Amplification-Free Measurement of DNA Lengths
AbstractAs biosensing devices shrink smaller and smaller, they approach a scale in which single molecule electronic sensing becomes possible. Here, we review the operation of single-enzyme transistors made using single-walled carbon nanotubes. These novel hybrid devices transduce the motions and catalytic activity of a single protein into an electronic signal for real-time monitoring of the protein’s activity. Analysis of these electronic signals reveals new insights into enzyme function and proves the electronic technique to be complementary to other single-molecule methods based on fluorescence. As one example of the nanocircuit technique, we have studied the Klenow Fragment (KF) of DNA polymerase I as it catalytically processes single-stranded DNA templates. The fidelity of DNA polymerases makes them a key component in many DNA sequencing techniques, and here we demonstrate that KF nanocircuits readily resolve DNA polymerization with single-base sensitivity. Consequently, template lengths can be directly counted from electronic recordings of KF’s base-by-base activity. After measuring as few as 20 copies, the template length can be determined with <1 base pair resolution, and different template lengths can be identified and enumerated in solutions containing template mixtures. View Full-Text
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Gül, O.T.; Pugliese, K.M.; Choi, Y.; Sims, P.C.; Pan, D.; Rajapakse, A.J.; Weiss, G.A.; Collins, P.G. Single Molecule Bioelectronics and Their Application to Amplification-Free Measurement of DNA Lengths. Biosensors 2016, 6, 29.
Gül OT, Pugliese KM, Choi Y, Sims PC, Pan D, Rajapakse AJ, Weiss GA, Collins PG. Single Molecule Bioelectronics and Their Application to Amplification-Free Measurement of DNA Lengths. Biosensors. 2016; 6(3):29.Chicago/Turabian Style
Gül, O. T.; Pugliese, Kaitlin M.; Choi, Yongki; Sims, Patrick C.; Pan, Deng; Rajapakse, Arith J.; Weiss, Gregory A.; Collins, Philip G. 2016. "Single Molecule Bioelectronics and Their Application to Amplification-Free Measurement of DNA Lengths." Biosensors 6, no. 3: 29.
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