High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide
Abstract
:1. Introduction
2. Experimental Section
2.1. Reagents and Proteins
2.2. Fabrication of an Optical Interference Mirror Slide
2.3. Preparation of Rhodamine B
2.4. Detection of Streptavidin
2.5. Optical System
2.6. Design of the Al2O3 Thickness
3. Results and Discussion
3.1. Polarizations of the Back-Scattered Excitation Light and Fluorescence Emission
3.2. Evaluation of the Image Contrast for Rhodamine B
3.3. High-Contrast Fluorescence Microscopy for Biomolecular Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yasuda, M.; Akimoto, T. High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide. Biosensors 2014, 4, 513-522. https://doi.org/10.3390/bios4040513
Yasuda M, Akimoto T. High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide. Biosensors. 2014; 4(4):513-522. https://doi.org/10.3390/bios4040513
Chicago/Turabian StyleYasuda, Mitsuru, and Takuo Akimoto. 2014. "High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide" Biosensors 4, no. 4: 513-522. https://doi.org/10.3390/bios4040513