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Biosensors 2013, 3(3), 312-326; doi:10.3390/bios3030312

A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform

Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA
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Received: 24 July 2013 / Revised: 28 August 2013 / Accepted: 5 September 2013 / Published: 13 September 2013
(This article belongs to the Special Issue Nanomaterials for Biodetection and Drug Delivery)
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Abstract

Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.
Keywords: surface enhanced Raman scattering; self-referencing; microorganism; nanoparticles surface enhanced Raman scattering; self-referencing; microorganism; nanoparticles
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Xiao, N.; Wang, C.; Yu, C. A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform. Biosensors 2013, 3, 312-326.

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