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Biosensors 2012, 2(4), 433-447; doi:10.3390/bios2040433
Article

A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well

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1 Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France 2 Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, n°1 avenue de l'Hôpital, 4000 Liège, Belgium 3 Centre Mémoire de Ressources et Recherche, Laboratoire des Maladies à Prions, Groupement Hospitalier Est; Hôpitaux de Lyon 59 bd Pinel, 69677 Bron cedex, France CTTM, 20 rue Thalès de Milet 72000 Le Mans, France 4 CTTM, 20 rue Thalès de Milet 72000 Le Mans, France
* Author to whom correspondence should be addressed.
Received: 13 September 2012 / Revised: 10 October 2012 / Accepted: 15 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Immunosensors 2012)
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Abstract

This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
Keywords: amphiphilic molecules; cold plasma; ELISA titration; prion protein amphiphilic molecules; cold plasma; ELISA titration; prion protein
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Mille, C.; Debarnot, D.; Zorzi, W.; Moualij, B.E.; Quadrio, I.; Perret-Liaudet, A.; Coudreuse, A.; Legeay, G.; Poncin-Epaillard, F. A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well. Biosensors 2012, 2, 433-447.

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