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Biosensors 2012, 2(4), 433-447; doi:10.3390/bios2040433
Article

A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well

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Received: 13 September 2012; in revised form: 10 October 2012 / Accepted: 15 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Immunosensors 2012)
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Abstract: This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
Keywords: amphiphilic molecules; cold plasma; ELISA titration; prion protein amphiphilic molecules; cold plasma; ELISA titration; prion protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mille, C.; Debarnot, D.; Zorzi, W.; Moualij, B.E.; Quadrio, I.; Perret-Liaudet, A.; Coudreuse, A.; Legeay, G.; Poncin-Epaillard, F. A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well. Biosensors 2012, 2, 433-447.

AMA Style

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F. A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well. Biosensors. 2012; 2(4):433-447.

Chicago/Turabian Style

Mille, Caroline; Debarnot, Dominique; Zorzi, Willy; Moualij, Benaissa E.; Quadrio, Isabelle; Perret-Liaudet, Armand; Coudreuse, Arnaud; Legeay, Gilbert; Poncin-Epaillard, Fabienne. 2012. "A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well." Biosensors 2, no. 4: 433-447.

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