Biosensors 2012, 2(4), 433-447; doi:10.3390/bios2040433
Article

A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well

Caroline Mille 1 email, Dominique Debarnot 1 email, Willy Zorzi 2 email, Benaissa El Moualij 2 email, Isabelle Quadrio 3 email, Armand Perret-Liaudet 3 , Arnaud Coudreuse 4 email, Gilbert Legeay 4 email and Fabienne Poncin-Epaillard 1,* email
1 Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France 2 Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, n°1 avenue de l'Hôpital, 4000 Liège, Belgium 3 Centre Mémoire de Ressources et Recherche, Laboratoire des Maladies à Prions, Groupement Hospitalier Est; Hôpitaux de Lyon 59 bd Pinel, 69677 Bron cedex, France CTTM, 20 rue Thalès de Milet 72000 Le Mans, France 4 CTTM, 20 rue Thalès de Milet 72000 Le Mans, France
* Author to whom correspondence should be addressed.
Received: 13 September 2012; in revised form: 10 October 2012 / Accepted: 15 October 2012 / Published: 24 October 2012
(This article belongs to the Special Issue Immunosensors 2012)
PDF Full-text Download PDF Full-Text [392 KB, uploaded 24 October 2012 14:36 CEST]
Abstract: This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
Keywords: amphiphilic molecules; cold plasma; ELISA titration; prion protein

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Mille, C.; Debarnot, D.; Zorzi, W.; Moualij, B.E.; Quadrio, I.; Perret-Liaudet, A.; Coudreuse, A.; Legeay, G.; Poncin-Epaillard, F. A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well. Biosensors 2012, 2, 433-447.

AMA Style

Mille C, Debarnot D, Zorzi W, Moualij BE, Quadrio I, Perret-Liaudet A, Coudreuse A, Legeay G, Poncin-Epaillard F. A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well. Biosensors. 2012; 2(4):433-447.

Chicago/Turabian Style

Mille, Caroline; Debarnot, Dominique; Zorzi, Willy; Moualij, Benaissa E.; Quadrio, Isabelle; Perret-Liaudet, Armand; Coudreuse, Arnaud; Legeay, Gilbert; Poncin-Epaillard, Fabienne. 2012. "A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well." Biosensors 2, no. 4: 433-447.

Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert