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Biosensors 2012, 2(3), 291-304; doi:10.3390/bios2030291

Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

1
Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain
2
Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain
3
Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain
*
Author to whom correspondence should be addressed.
Received: 11 July 2012 / Revised: 2 August 2012 / Accepted: 13 August 2012 / Published: 14 August 2012
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Abstract

In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).
Keywords: micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ortega, F.J.; Bañuls, M.-J.; Sanza, F.J.; Casquel, R.; Laguna, M.F.; Holgado, M.; López-Romero, D.; Barrios, C.A.; Maquieira, Á.; Puchades, R. Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors 2012, 2, 291-304.

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