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Biosensors 2012, 2(3), 291-304; doi:10.3390/bios2030291
Article

Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars

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Received: 11 July 2012; in revised form: 2 August 2012 / Accepted: 13 August 2012 / Published: 14 August 2012
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Abstract: In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).
Keywords: micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ortega, F.J.; Bañuls, M.-J.; Sanza, F.J.; Casquel, R.; Laguna, M.F.; Holgado, M.; López-Romero, D.; Barrios, C.A.; Maquieira, Á.; Puchades, R. Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors 2012, 2, 291-304.

AMA Style

Ortega FJ, Bañuls M-J, Sanza FJ, Casquel R, Laguna MF, Holgado M, López-Romero D, Barrios CA, Maquieira Á, Puchades R. Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars. Biosensors. 2012; 2(3):291-304.

Chicago/Turabian Style

Ortega, Francisco J.; Bañuls, María-José; Sanza, Francisco J.; Casquel, Rafael; Laguna, María Fe; Holgado, Miguel; López-Romero, David; Barrios, Carlos A.; Maquieira, Ángel; Puchades, Rosa. 2012. "Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars." Biosensors 2, no. 3: 291-304.

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