Biosensors 2011, 1(3), 107-117; doi:10.3390/bios1030107
Article

Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases

1email, 2email, 1email and 1,* email
Received: 15 May 2011; in revised form: 15 June 2011 / Accepted: 1 July 2011 / Published: 12 July 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0–544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.
Keywords: liver diseases; alanine aminotransferase; electrochemical detection; biosensor
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MDPI and ACS Style

Hsueh, C.-J.; Wang, J.H.; Dai, L.; Liu, C.-C. Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases. Biosensors 2011, 1, 107-117.

AMA Style

Hsueh C-J, Wang JH, Dai L, Liu C-C. Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases. Biosensors. 2011; 1(3):107-117.

Chicago/Turabian Style

Hsueh, Chang-Jung; Wang, Joanne H.; Dai, Liming; Liu, Chung-Chiun. 2011. "Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases." Biosensors 1, no. 3: 107-117.

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