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Biosensors 2011, 1(3), 107-117; doi:10.3390/bios1030107
Article

Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases

1
,
2
,
1
 and
1,*
1 Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA 2 Department of Biology, Brown University, 69 Brown Street, Providence, RI 02912, USA
* Author to whom correspondence should be addressed.
Received: 15 May 2011 / Revised: 15 June 2011 / Accepted: 1 July 2011 / Published: 12 July 2011
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Abstract

Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0–544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.
Keywords: liver diseases; alanine aminotransferase; electrochemical detection; biosensor liver diseases; alanine aminotransferase; electrochemical detection; biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hsueh, C.-J.; Wang, J.H.; Dai, L.; Liu, C.-C. Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases. Biosensors 2011, 1, 107-117.

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