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Challenges 2018, 9(1), 5; https://doi.org/10.3390/challe9010005

The Dichotomy of the Poly(ADP-Ribose) Polymerase-Like Thermozyme from Sulfolobus solfataricus

1
Department of Biology, University of Naples “Federico II”, 80126 Naples, Italy
2
National Institute of Biostructures and Biosystems (INBB), via delle Medaglie d’Oro, 00136 Rome, Italy
Received: 13 December 2017 / Revised: 28 January 2018 / Accepted: 30 January 2018 / Published: 31 January 2018
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Abstract

The first evidence of an ADP-ribosylating activity in Archaea was obtained in Sulfolobus solfataricus(strain MT-4) where a poly(ADP-ribose) polymerase (PARP)-like thermoprotein, defined with the acronymous PARPSso, was found. Similarly to the eukaryotic counterparts PARPSso cleaves beta-nicotinamide adenine dinucleotide to synthesize oligomers of ADP-ribose; cross-reacts with polyclonal anti-PARP-1 catalytic site antibodies; binds DNA. The main differences rely on the molecular mass (46.5 kDa) and the thermophily of PARPSso which works at 80 °C. Despite the biochemical properties that allow correlating it to PARP enzymes, the N-terminal and partial amino acid sequences available suggest that PARPSso belongs to a different group of enzymes, the DING proteins, an item discussed in detail in this review.This finding makes PARPSso the first example of a DING protein in Archaea and extends the existence of DING proteins into all the biological kingdoms. PARPSsohas a cell peripheral localization, along with the edge of the cell membrane. The ADP-ribosylation reaction is reverted by a poly(ADP-ribose) glycohydrolase-like activity, able to use the eukaryotic poly(ADP-ribose) as a substrate too. Here we overview the research of (ADP-ribosyl)ation in Sulfolobus solfataricus in the past thirty years and discuss the features of PARPSso common with the canonical poly(ADP-ribose) polymerases, and the structure fitting with that of DING proteins. View Full-Text
Keywords: Archaea; Crenarcheota; DING proteins; PARG; PARP; PARPSso; poly(ADP-ribosyl)ation; Sulfolobus solfataricus Archaea; Crenarcheota; DING proteins; PARG; PARP; PARPSso; poly(ADP-ribosyl)ation; Sulfolobus solfataricus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Faraone Mennella, M.R. The Dichotomy of the Poly(ADP-Ribose) Polymerase-Like Thermozyme from Sulfolobus solfataricus. Challenges 2018, 9, 5.

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