Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cloning and Purification of Recombinant AtDJ-1B
2.2. Circular Dichroism (CD)
2.3. Thermal Unfolding
2.4. Glyoxalase Assay
2.5. Chaperone Assay
2.6. Oxidation of AtDJ-1B for Mass Spectrometric Analysis
2.7. Determination of Cysteine Oxidation States by Mass Spectrometry
2.8. Gene Expression Levels
2.9. Plant Material
2.10. RT-qPCR
2.11. Growth Conditions and Plant Stress Assays
3. Results
3.1. AtDJ-1B Contains Multiple Oxidant-Sensitive Cysteines
3.2. Oxidized AtDJ-1B Becomes More Thermostable
3.3. Oxidation Inactivates the Glyoxalase Activity of AtDJ-1B
3.4. Oxidation does not Affect the Chaperone Activity of AtDJ-1B
3.5. DJ-1B-Deficient Plants are Phenotypically Identical to Wildtype
3.6. Transcript Levels of DJ-1B are Stress-Independent
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lewandowska, A.; Vo, T.N.; Nguyen, T.-D.H.; Wahni, K.; Vertommen, D.; Van Breusegem, F.; Young, D.; Messens, J. Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2. Antioxidants 2019, 8, 8. https://doi.org/10.3390/antiox8010008
Lewandowska A, Vo TN, Nguyen T-DH, Wahni K, Vertommen D, Van Breusegem F, Young D, Messens J. Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2. Antioxidants. 2019; 8(1):8. https://doi.org/10.3390/antiox8010008
Chicago/Turabian StyleLewandowska, Aleksandra, Trung Nghia Vo, Thuy-Dung Ho Nguyen, Khadija Wahni, Didier Vertommen, Frank Van Breusegem, David Young, and Joris Messens. 2019. "Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2" Antioxidants 8, no. 1: 8. https://doi.org/10.3390/antiox8010008