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Antioxidants 2018, 7(3), 34; https://doi.org/10.3390/antiox7030034

Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.

1
Programa Institucional de Doctorado en Ciencias Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58240, Mich., Mexico
2
Facultad de Químico Farmacobiología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58240, Mich., México
3
Facultad de Ingeniería Civil, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58240, Mich., México
4
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Centro de Investigación Regional Norte-Centro, Campo Experimental Pabellón, Pabellón de Arteaga 20660, Aguascalientes, Mexico
5
Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mich., Mexico
6
Departamento de Bioquímica, Instituto Tecnológico de Morelia, Morelia 58120, Mich., Mexico
*
Author to whom correspondence should be addressed.
Received: 6 January 2018 / Revised: 14 February 2018 / Accepted: 22 February 2018 / Published: 27 February 2018
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Abstract

Guava leaf (Psidium guajava L.) extracts are used in both traditional medicine and the pharmaceutical industry. The antioxidant compounds in P. guajava leaves can have positive effects including anti-inflammatory, anti-hyperglycemic, hepatoprotective, analgesic, anti-cancer effects, as well as protecting against cardiovascular diseases. In the present study, phenolic compounds and in vitro antioxidant capacity were measured in extracts obtained with polar and non-polar solvents from leaves of two varieties of guava, Calvillo Siglo XXI and Hidrozac. The quantity of total phenolics and total flavonoids were expressed as equivalents of gallic acid and quercetin, respectively. Hydroxyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity using fluorescein (ORAC-FL) in vitro tests were used to assess the radical scavenging abilities of the extracts. The total phenolics were higher in the aqueous fraction of the variety Calvillo Siglo XXI, while in the Hidrozac variety total phenolics were higher in the acetone and chloroform fractions. Total flavonoids were higher in all fractions in the variety Calvillo Siglo XXI. Total phenolics showed a highly positive correlation for ORAC-FL, and a moderately positive correlation with hydroxyl radicals. Finally, total flavonoids showed a slightly positive correlation for ORAC-FL and hydroxyl radicals. Both varieties of guava leaf extract showed excellent antioxidant properties. View Full-Text
Keywords: Psidium guajava; ORAC; DPPH; ABTS; total polyphenols; total flavonoids; nano spray-dryer Psidium guajava; ORAC; DPPH; ABTS; total polyphenols; total flavonoids; nano spray-dryer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Camarena-Tello, J.C.; Martínez-Flores, H.E.; Garnica-Romo, M.G.; Padilla-Ramírez, J.S.; Saavedra-Molina, A.; Alvarez-Cortes, O.; Bartolomé-Camacho, M.C.; Rodiles-López, J.O. Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.. Antioxidants 2018, 7, 34.

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