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Antioxidants 2014, 3(4), 745-757; doi:10.3390/antiox3040745

Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts

1
Faculty of Agricultural Sciences, University Federal of Grande Dourados (UFGD), 79804-970/533 Dourados-MS, Brazil
2
Biological and Environmental Sciences, University Federal of Grande Dourados (UFGD), 79804-970/533 Dourados-MS, Brazil
3
Chemistry Program, State University of Mato Grosso do Sul (UEMS), Mato Grosso do Sul, 79804-970/351 Dourados-MS, Brazil
*
Author to whom correspondence should be addressed.
Received: 16 May 2014 / Revised: 23 July 2014 / Accepted: 8 September 2014 / Published: 10 November 2014
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Abstract

The antioxidant activity of Psychotria carthagenensis, P. leiocarpa, P. capillacea and P. deflexa (Rubiaceae) extracts were investigated, and the concentrations of total phenolics, flavonoids, condensed tannins and flavonols were determined. The chemical compositions of the extracts were investigated using the high performance liquid chromatography (HPLC/PAD) method. We used 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), β-Carotene bleaching and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cations to determine antioxidant activity. The ability to scavenge radical was measured in these experiments by the discoloration of the solution. Concentrations of constituents were measured spectrophotometrically. P. carthagenensis and P. capillacea exhibited the highest antioxidant activity, in the DPPH test, β-carotene bleaching and ABTS system. The highest phenolic, flavonoid, condensed tannin and flavonol concentration was found in P. carthagenensis and P. capillacea extracts. HPLC-PDA analysis of P. carthagenensis and P. capillacea revealed hydroxycinnamic acid (p-coumaric acid). This is the first report on the antioxidant properties and constituent analysis of these Psychotria extracts. View Full-Text
Keywords: Psychotria; antioxidant activity; p-coumaric acid Psychotria; antioxidant activity; p-coumaric acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Formagio, A.S.N.; Volobuff, C.R.F.; Santiago, M.; Cardoso, C.A.L.; Vieira, M.C.; Valdevina Pereira, Z. Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants 2014, 3, 745-757.

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