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Antioxidant, Iron Chelating and Tyrosinase Inhibitory Activities of Extracts from Talinum triangulare Leach Stem
Department of Chemistry, Institute of Exact Sciences, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil
Department of Botanic, Institute of Biology, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil
* Author to whom correspondence should be addressed.
Received: 11 May 2013; in revised form: 14 June 2013 / Accepted: 28 June 2013 / Published: 17 July 2013
Abstract: The aim of this work is to evaluate the antioxidant activity against the radical species DPPH, the reducing capacity against Fe II ions, and the inhibitory activity on the tyrosinase enzyme of the T. triangulare. Hydromethanolic crude extract provided two fractions after the liquid/liquid partition with chloroform. The Folin-Ciocalteu method determined the total phenolic content of the crude extract (CE) and the hydromethanolic fraction (Fraction 1), resulting in a concentration of 0.5853 g/100 g for Fraction 1, and 0.1400 g/100 g for the CE. Taking into account the results of the DPPH, the free radical scavenging capacity was confirmed. The formation of complexes with Fe II ions was evaluated by UV/visible spectrometry; results showed that CE has complexing power similar to the positive control (Gingko biloba extract).The inhibitory capacity of samples against the tyrosinase enzyme was determined by the oxidation of L-DOPA, providing IC50 values of 13.3 μg·mL−1 (CE) and 6.6 μg·mL−1 (Fraction 1). The values indicate that Fraction 1 was more active and showed a higher inhibitory power on the tyrosinase enzyme than the ascorbic acid, used as positive control. The hydromethanolic extract of T. triangulare proved to have powerful antioxidant activity and to inhibit the tyrosinase enzyme; its potential is increased after the partition with chloroform.
Keywords: portulacaceae; DPPH; Fe II ions; oxidase; tyrosinase
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Oliveira Amorim, A.P.; Campos de Oliveira, M.C.; de Azevedo Amorim, T.; Echevarria, A. Antioxidant, Iron Chelating and Tyrosinase Inhibitory Activities of Extracts from Talinum triangulare Leach Stem. Antioxidants 2013, 2, 90-99.
Oliveira Amorim AP, Campos de Oliveira MC, de Azevedo Amorim T, Echevarria A. Antioxidant, Iron Chelating and Tyrosinase Inhibitory Activities of Extracts from Talinum triangulare Leach Stem. Antioxidants. 2013; 2(3):90-99.
Oliveira Amorim, Ana P.; Campos de Oliveira, Márcia C.; de Azevedo Amorim, Thiago; Echevarria, Aurea. 2013. "Antioxidant, Iron Chelating and Tyrosinase Inhibitory Activities of Extracts from Talinum triangulare Leach Stem." Antioxidants 2, no. 3: 90-99.