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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2015, 4(1), 41-52; doi:10.3390/microarrays4010041

Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers)

1,2,3
,
1,2,3
and
1,2,3,*
1
Univ. Grenoble Alpes, INAC-SPRAM, F-38000 Grenoble, France
2
CEA, INAC-SPrAM, F-38000 Grenoble, France
3
CNRS, INAC-SPrAM, F-38000 Grenoble, France
*
Author to whom correspondence should be addressed.
Academic Editor: Ulrich Certa
Received: 10 December 2014 / Accepted: 2 February 2015 / Published: 9 February 2015
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Abstract

The detection of small molecules by biosensors remains a challenge for diagnostics in many areas like pharmacology, environment or homeland security. The main difficulty comes from both the low molecular weight and low concentrations of most targets, which generally requires an indirect detection with an amplification or a sandwich procedure. In this study, we combine both strategies as the amplification of Surface Plasmon Resonance imaging (SPRi) signal is obtained by the use of gold nanoparticles and the sequence engineering of split-aptamers, short oligonucleotides strands with strong affinity towards small targets, allows for a sandwich structure. Combining those two strategies, we obtained state-of-the-art results in the limit of detection (LOD = 50 nM) with the model target adenosine. Furthermore, the SPRi detection led on aptamer microarrays paves the way for potential multi-target detections thanks to the multi-probe imaging approach. View Full-Text
Keywords: split-aptamers; Surface Plasmon Resonance imaging; gold nanoparticles; small molecules detection split-aptamers; Surface Plasmon Resonance imaging; gold nanoparticles; small molecules detection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Melaine, F.; Roupioz, Y.; Buhot, A. Gold Nanoparticles Surface Plasmon Resonance Enhanced Signal for the Detection of Small Molecules on Split-Aptamer Microarrays (Small Molecules Detection from Split-Aptamers). Microarrays 2015, 4, 41-52.

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