Long-Term Eutrophication and Dynamics of Bloom-Forming Microbial Communities during Summer HAB in Large Arctic Lake
Abstract
:1. Introduction
2. Materials and Methods
2.1. Hydrochemical Analysis
2.2. Prediction of Chlorophyll-a Concentrations and Visualization of TC/TN/TP/Chl-a Ratios
2.3. DNA Preparation and Processing
2.4. Analysis of Bacterial Communities
2.5. Analysis of the Publically Available Dolichospermum Genomes
3. Results
3.1. Eutrophication Dynamics
3.2. Nutrient Stoichiometry and Chlorophyll-a
3.3. Microbial Communities
3.4. Putative Genetic Triats Beneath Simultaneous Blooming
4. Discussion
4.1. Selection of the Primers
4.2. Concentrations of Macronutrients and Chlorophyll-a
4.3. HABs in Arctic Waters
4.4. Putative Genetic Triats and Adaptations Beneath Simultaneous Blooming
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hmax (m) | Z (m) | Slake (km2) | V (km3) | Scathc (km2) | R (m/s) | t (Year) | CLP (mg P/m2; Year) | Q(P)max (ton P; Yyear) | M(P) (mg P/m2; Year) | L(P) (ton P; Year) | |
---|---|---|---|---|---|---|---|---|---|---|---|
Entire Lake | 67 | 13.3 | 813 | 10.81 | 12300 | 145 | 2.38 | 333 | 270 | 22 | 1507 |
Bolshaya I. | 67 | 14.7 | 312 | 4.58 | 4993 | 145 | 1.00 | 368 | 115 | 23 | 999 |
Jokostrovskaya I | 42 | 10.9 | 352 | 3.84 | 6070 | 145 | 0.84 | 273 | 96 | 16 | 332 |
Babinskaya I. | 43.5 | 16.3 | 149 | 2.42 | 1238 | 145 | 0.53 | 408 | 61 | 49 | 176 |
pH | NH4 (µgN/l) | NO3 (µgN/) | NO3: NH3 | TN (µgN/l) | Nopr (µgN/l) | PO4 (µgP/l) | TP (µgP/l) | TN:TP | |
---|---|---|---|---|---|---|---|---|---|
Bolshaya I. | 7.5 6.8–8.4 | 7.0 1.0–114 | 5.0 0–1156 | 3.4 0–39.0 | 196 119–1629 | 188 117–365 | 3.0 1.0–153 | 27.0 7.0–251 | 8.4 2.5–18.9 |
Jokostrovskaya I. | 7.3 7.1–7.4 | 10.0 1.0–58.0 | 2.0 0–30.0 | 1.0 0–12.0 | 152 111–259 | 138 84–199 | 1.0 0–2.0 | 7.0 5.0–12.0 | 21.6 14.7–34.0 |
Babinskaya I. | 7.3 7.0–7.5 | 9.0 2.0–22.0 | 7.0 0–78.0 | 2.8 0–21.7 | 156 66.0–318 | 126 44–250 | 1.0 0–3.0 | 5.0 3.0–22.0 | 30.6 5.7–63.6 |
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Kashulin, N.; Kashulina, T.; Bekkelund, A. Long-Term Eutrophication and Dynamics of Bloom-Forming Microbial Communities during Summer HAB in Large Arctic Lake. Environments 2021, 8, 82. https://doi.org/10.3390/environments8080082
Kashulin N, Kashulina T, Bekkelund A. Long-Term Eutrophication and Dynamics of Bloom-Forming Microbial Communities during Summer HAB in Large Arctic Lake. Environments. 2021; 8(8):82. https://doi.org/10.3390/environments8080082
Chicago/Turabian StyleKashulin, Nikolay, Tatiana Kashulina, and Alexander Bekkelund. 2021. "Long-Term Eutrophication and Dynamics of Bloom-Forming Microbial Communities during Summer HAB in Large Arctic Lake" Environments 8, no. 8: 82. https://doi.org/10.3390/environments8080082