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Microorganisms 2018, 6(1), 14; doi:10.3390/microorganisms6010014

Bacterial Microbiota of Rice Roots: 16S-Based Taxonomic Profiling of Endophytic and Rhizospheric Diversity, Endophytes Isolation and Simplified Endophytic Community

1
Bacteriology Group, International Center for Genetic Engineering and Biotechnology ICGEB, 34149 Trieste, Italy
2
Laboratory of Plant Biotechnology and Virology, Center for Microbiology and Cell Biology, Venezuelan Institute of Scientific Research IVIC, Caracas 1020A, Venezuela
3
Department of Life Science, University of Trieste, 34127 Trieste, Italy
*
Author to whom correspondence should be addressed.
Received: 20 November 2017 / Revised: 23 January 2018 / Accepted: 8 February 2018 / Published: 11 February 2018
(This article belongs to the Special Issue Symbiotic Plant-Bacterial Endospheric Interactions)
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Abstract

Rice is currently the most important food crop in the world and we are only just beginning to study the bacterial associated microbiome. It is of importance to perform screenings of the core rice microbiota and also to develop new plant-microbe models and simplified communities for increasing our understanding about the formation and function of its microbiome. In order to begin to address this aspect, we have performed a 16S rDNA taxonomic bacterial profiling of the rhizosphere and endorhizosphere of two high-yield rice cultivars—Pionero 2010 FL and DANAC SD20A—extensively grown in Venezuela in 2014. Fifteen putative bacterial endophytes were then isolated from surface-sterilized roots and further studied in vitro and in planta. We have then performed inoculation of rice seedlings with a simplified community composed by 10 of the isolates and we have tracked them in the course of 30 days in greenhouse cultivation. The results obtained suggest that a set was able to significantly colonize together the rice endorhizospheres, indicating possible cooperation and the ability to form a stable multispecies community. This approach can be useful in the development of microbial solutions for a more sustainable rice production. View Full-Text
Keywords: rice; endophyte; sustainable agriculture; plant microbiome; simplified bacterial community; syncom; taxonomic profiling; core plant microbiome rice; endophyte; sustainable agriculture; plant microbiome; simplified bacterial community; syncom; taxonomic profiling; core plant microbiome
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Moronta-Barrios, F.; Gionechetti, F.; Pallavicini, A.; Marys, E.; Venturi, V. Bacterial Microbiota of Rice Roots: 16S-Based Taxonomic Profiling of Endophytic and Rhizospheric Diversity, Endophytes Isolation and Simplified Endophytic Community. Microorganisms 2018, 6, 14.

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