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Microorganisms 2017, 5(3), 53; doi:10.3390/microorganisms5030053

Multiple Antibiotic-Resistant, Extended Spectrum-β-Lactamase (ESBL)-Producing Enterobacteria in Fresh Seafood

Quality Control Laboratory, Post Harvest Technology Department, Central Institute of Fisheries Education (CIFE), Mumbai 400061, India
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Received: 22 June 2017 / Revised: 16 August 2017 / Accepted: 25 August 2017 / Published: 30 August 2017
(This article belongs to the Section Environmental Microbiology)
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Abstract

Members of the family Enterobacteriaceae include several human pathogens that can be acquired through contaminated food and water. In this study, the incidence of extended spectrum β-lactamase (ESBL)-producing enterobacteria was investigated in fresh seafood sold in retail markets. The ESBL-positive phenotype was detected in 169 (78.60%) isolates, with Escherichia coli being the predominant species (53), followed by Klebsiella oxytoca (27), and K. pneumoniae (23). More than 90% of the isolates were resistant to third generation cephalosporins, cefotaxime, ceftazidime, and cefpodoxime. Sixty-five percent of the isolates were resistant to the monobactam drug aztreonam, 40.82% to ertapenem, and 31.36% to meropenem. Resistance to at least five antibiotics was observed in 38.46% of the isolates. Polymerase Chain Reaction (PCR) analysis of ESBL-encoding genes detected blaCTX, blaSHV, and blaTEM genes in 76.92%, 63.3%, and 44.37% of the isolates, respectively. Multiple ESBL genes were detected in majority of the isolates. The recently discovered New Delhi metallo-β-lactamase gene (blaNDM-1) was detected in two ESBL+ isolates. Our study shows that secondary contamination of fresh seafood with enteric bacteria resistant to multiple antibiotics may implicate seafood as a potential carrier of antibiotic resistant bacteria and emphasizes an urgent need to prevent environmental contamination and dissemination of such bacteria. View Full-Text
Keywords: seafood; enterobacteria; ESBL; blaNDM; antibiotic resistance seafood; enterobacteria; ESBL; blaNDM; antibiotic resistance
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Sanjit Singh, A.; Lekshmi, M.; Prakasan, S.; Nayak, B.B.; Kumar, S. Multiple Antibiotic-Resistant, Extended Spectrum-β-Lactamase (ESBL)-Producing Enterobacteria in Fresh Seafood. Microorganisms 2017, 5, 53.

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