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Microorganisms 2015, 3(1), 94-112; doi:10.3390/microorganisms3010094

Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

1
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA
2
Department of Microbiology, University of Washington, Seattle, WA 98195, USA
3
Science Institute, University of Washington, Seattle, WA 98195, USA
4
DOE Joint Genome Institute, Walnut Creek, CA 94598, USA
Present address: Department of Chemical Engineering and Materials Science, University of California Irvine, Irvine, CA 92697, USA.
Present address: Denali Advanced Integration, Redmond, WA 98052, USA.
§
Present address: Biology Department, San Diego State University, San Diego, CA 92182, USA.
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew McDowell
Received: 3 February 2015 / Revised: 27 February 2015 / Accepted: 6 March 2015 / Published: 20 March 2015
(This article belongs to the Special Issue Microbial C1 Metabolism)
View Full-Text   |   Download PDF [688 KB, uploaded 20 March 2015]   |  

Abstract

Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria. View Full-Text
Keywords: methylotrophy; genomics; Arthrobacter; Bacillus; Mycobacterium; Lake Washington methylotrophy; genomics; Arthrobacter; Bacillus; Mycobacterium; Lake Washington
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

McTaggart, T.L.; Beck, D.A.C.; Setboonsarng, U.; Shapiro, N.; Woyke, T.; Lidstrom, M.E.; Kalyuzhnaya, M.G.; Chistoserdova, L. Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria. Microorganisms 2015, 3, 94-112.

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