Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Nosocomial Isolates and Reference Control Strain
2.2. Inoculum Adjustment
2.3. Experimental Strategy
2.4. Sterility Compendial Method
2.5. Automated Blood Culture Methods
2.6. Subculture
2.7. Parameters under Investigation
2.8. Specificity
2.9. Detection Limit
2.10. Ruggedness and Repeatability
2.11. Technical Cross-Contamination
2.12. Comparative Data Analysis
3. Results
3.1. Specificity
3.2. Detection Limit
3.3. Ruggedness and Repeatability
3.4. Technical Cross-Contaminations
3.5. Comparative Data Analisys
3.5.1. Comparative Analysis of the Growth Promotion Test (GPT) for the Three Systems
3.5.2. Time to Detection (TTD)
3.6. Recovery of Aspergillus spp. on Solid Culture Medium
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Clinical Isolates | Reference Strain |
---|---|
Aspergillus fumigatus | Aspergillus brasiliensis ATCC 16404 |
Aspergillus flavus Aspergillus niger Aspergillus terreus |
Aspergillus | Bottle Media | Recovery | Recovery TTD (h) ± SD |
---|---|---|---|
A. brasiliensis (ATCC 16404) | TSB | 12/12 | 80 ± 15.6 |
iAST | 12/12 | 58.4 ± 4,8 | |
Aerobic/F | 12/12 | 68 ± 9.3 | |
Mycosis-IC/F | 12/12 | 68 ± 9.3 | |
A. niger | TSB | 12/12 | 79.5 ± 11.3 |
iAST | 12/12 | 51.6 ± 4.4 | |
Aerobic/F | 12/12 | 49.2 ± 3.1 | |
Mycosis-IC/F | 12/12 | 51.6 ± 4.7 | |
A. fumigatus | TSB | 12/12 | 84 ± 12.5 |
iAST | 12/12 | 34.8 ± 2.6 | |
Aerobic/F | 12/12 | 35.8 ± 3.5 | |
Mycosis-IC/F | 12/12 | 35.8 ± 2.8 | |
A. flavus | TSB | 12/12 | 87.2 ± 12.1 |
iAST | 12/12 | 41.54 ± 4.8 | |
Aerobic/F | 12/12 | 48.9 ± 6 | |
Mycosis-IC/F | 12/12 | 52.6 ± 5.4 | |
A. terrus | TSB | 12/12 | 104 ± 11.8 |
iAST | 12/12 | 42 ± 4.9 | |
Aerobic/F | 12/12 | 38.3 ± 3.7 | |
Mycosis-IC/F | 12/12 | 39.6 ± 3.2 |
Range of Total CFU Inoculated | 25–50 | 5–10 | 2–5 | 1–2 | 0–1 | |
---|---|---|---|---|---|---|
Aspergillus | Bottle Media | Recovery | Recovery | Recovery | Recovery | Recovery |
A. brasiliensis (ATCC 16404) | TSB | 12/12 | 12/12 | 11/12 | 9/12 | 2/12 |
iAST | 12/12 | 12/12 | 12/12 | 9/12 | 0/12 | |
Aerobic/F | 12/12 | 12/12 | 10/12 | 10/12 | 1/12 | |
Mycosis-IC/F | 12/12 | 12/12 | 12/12 | 9/12 | 1/12 | |
A. niger | TSB | 12/12 | 12/12 | 12/12 | 10/12 | 1/12 |
iAST | 12/12 | 12/12 | 12/12 | 9/12 | 0/12 | |
Aerobic/F | 12/12 | 12/12 | 11/12 | 10/12 | 3/12 | |
Mycosis-IC/F | 12/12 | 12/12 | 10/12 | 8/12 | 0/12 | |
A. fumigatus | TSB | 12/12 | 12/12 | 12/12 | 9/12 | 0/12 |
iAST | 12/12 | 12/12 | 11/12 | 10/12 | 2/12 | |
Aerobic/F | 12/12 | 12/12 | 11/12 | 9/12 | 0/12 | |
Mycosis-IC/F | 12/12 | 12/12 | 11/12 | 10/12 | 1/12 | |
A. flavus | TSB | 12/12 | 12/12 | 11/12 | 9/12 | 2/12 |
iAST | 12/12 | 12/12 | 10/12 | 9/12 | 0/12 | |
Aerobic/F | 12/12 | 12/12 | 12/12 | 9/12 | 1/12 | |
Mycosis-IC/F | 12/12 | 12/12 | 10/12 | 10/12 | 1/12 | |
A. terrus | TSB | 12/12 | 12/12 | 10/12 | 9/12 | 2/12 |
iAST | 12/12 | 12/12 | 10/12 | 10/12 | 0/12 | |
Aerobic/F | 12/12 | 12/12 | 11/12 | 9/12 | 1/12 | |
Mycosis-IC/F | 12/12 | 12/12 | 10/12 | 10/12 | 1/12 |
Range of Total CFU Inoculated | 25–50 | ||||
---|---|---|---|---|---|
Aspergillus | Bottle Media | Positive Detection in Liquid Medium | Recovery from Solid Medium | ||
First Procedure 1 | Second Procedure 2 | Third Procedure 3 | |||
A. brasiliensis (ATCC 16404) | TSB | 12/12 | 1/12 | 2/12 | 12/12 |
iAST | 12/12 | 0/12 | 2/12 | 12/12 | |
Aerobic/F | 12/12 | 1/12 | 4/12 | 12/12 | |
Mycosis-IC/F | 12/12 | 0/12 | 3/12 | 12/12 | |
A. niger | TSB | 12/12 | 1/12 | 3/12 | 12/12 |
iAST | 12/12 | 0/12 | 3/12 | 12/12 | |
Aerobic/F | 12/12 | 2/12 | 2/12 | 12/12 | |
Mycosis-IC/F | 12/12 | 0/12 | 3/12 | 12/12 | |
A. fumigatus | TSB | 12/12 | 1/12 | 3/12 | 12/12 |
iAST | 12/12 | 1/12 | 2/12 | 12/12 | |
Aerobic/F | 12/12 | 0/12 | 4/12 | 12/12 | |
Mycosis-IC/F | 12/12 | 1/12 | 3/12 | 12/12 | |
A. flavus | TSB | 12/12 | 2/12 | 3/12 | 12/12 |
iAST | 12/12 | 0/12 | 2/12 | 12/12 | |
Aerobic/F | 12/12 | 0/12 | 2/12 | 12/12 | |
Mycosis-IC/F | 12/12 | 1/12 | 3/12 | 12/12 | |
A. terrus | TSB | 12/12 | 1/12 | 3/12 | 12/12 |
iAST | 12/12 | 1/12 | 2/12 | 12/12 | |
Aerobic/F | 12/12 | 0/12 | 2/12 | 12/12 | |
Mycosis-IC/F | 12/12 | 0/12 | 2/12 | 12/12 |
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Pasqua, S.; Monaco, F.; Cardinale, F.; Bonelli, S.; Conaldi, P.G.; D’Apolito, D. Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles. Microorganisms 2022, 10, 2026. https://doi.org/10.3390/microorganisms10102026
Pasqua S, Monaco F, Cardinale F, Bonelli S, Conaldi PG, D’Apolito D. Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles. Microorganisms. 2022; 10(10):2026. https://doi.org/10.3390/microorganisms10102026
Chicago/Turabian StylePasqua, Salvatore, Francesco Monaco, Francesca Cardinale, Simone Bonelli, Pier Giulio Conaldi, and Danilo D’Apolito. 2022. "Growth Performance and Recovery of Nosocomial Aspergillus spp. in Blood Culture Bottles" Microorganisms 10, no. 10: 2026. https://doi.org/10.3390/microorganisms10102026