Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Ethical Approval
2.3. Bacterial Isolation
2.4. Antimicrobial Susceptibility Testing (AST) Using VITEK 2
2.5. Whole-Genome Sequencing (WGS) and Analysis
3. Results
3.1. Bacterial Isolation
3.2. Antimicrobial Susceptibility Testing (AST)
3.3. Whole-Genome Sequence (WGS) Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cohort | Year | Sampling Date | Positives/Number of Samples Taken | Sample Type | Isolate No. (Origin of Sample) |
---|---|---|---|---|---|
1 | 2020 | 27 May | 1/15 | C | 274 (11) |
10 June | 1/15 | C | 273 (5) | ||
1 | F | 275 (pooled, non-duck) | |||
25 June | 1/15 | C | 285 (5) | ||
8 July | 6/15 | C | 277–282 (1–3, 9, 10, 13) | ||
1 | F | 283 (pooled) | |||
22 July | 0/15 | C | |||
5 August | 0/15 | C | |||
18 August | 0/15 | C | |||
1 September | 0/15 | C | |||
16 September | 0/15 | C | |||
30 September | 0/15 | C | |||
13 October | 0/15 | C | |||
11 November | 0/15 | C | |||
2 December | 0/15 | C | |||
22 December | 0/15 | C | |||
2021 | 19 January | 0/15 | C | ||
2 | 2021 | 3 June | 3/10 | C | 1600–1602 (1, 2, 10) |
1 | F | 1603 * (pooled) | |||
17 June | 0/10 | C | |||
8 July | 0/9 | C | |||
30 July | 0/9 | C | |||
26 August | 0/10 | C | |||
14 September | 0/12 | C | |||
26 October | 0/7 | C | |||
8/9 November | 0/5 | C | |||
17 November | 0/7 | C | |||
23 November | 0/6 | C | |||
15 December | 0/8 | C | |||
2022 | 14 January | 0/5 | C | ||
3 February | 0/7 | C | |||
24 February | 0/8 | C | |||
9 March | 0/4 | C | |||
March | 1/13 | C | 3303 (12) |
Cohort | Designation | AMO | AMP | AMO/CA | CFL | CTX | CAZ | CFZ/TAZ | FEP | IMI | MEM | AMI | GEN | TOB | CIP | TGC | FOS | COL | TMP/SMX | ESBL | MDR |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 273 | X | |||||||||||||||||||
274 | X | ||||||||||||||||||||
275 | X | X | |||||||||||||||||||
277 | X | ||||||||||||||||||||
278 | X | ||||||||||||||||||||
279 | X | ||||||||||||||||||||
280 | X | ||||||||||||||||||||
281 | X | ||||||||||||||||||||
282 | X | ||||||||||||||||||||
283 | X | ||||||||||||||||||||
285 | X | X | |||||||||||||||||||
2 | 1600 | X | |||||||||||||||||||
1601 | X | ||||||||||||||||||||
1602 | X | ||||||||||||||||||||
1603 | X | ||||||||||||||||||||
3303 | X |
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Dreyer, S.; Globig, A.; Bachmann, L.; Schütz, A.K.; Schaufler, K.; Homeier-Bachmann, T. Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany. Microorganisms 2022, 10, 1968. https://doi.org/10.3390/microorganisms10101968
Dreyer S, Globig A, Bachmann L, Schütz AK, Schaufler K, Homeier-Bachmann T. Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany. Microorganisms. 2022; 10(10):1968. https://doi.org/10.3390/microorganisms10101968
Chicago/Turabian StyleDreyer, Sylvia, Anja Globig, Lisa Bachmann, Anne K. Schütz, Katharina Schaufler, and Timo Homeier-Bachmann. 2022. "Longitudinal Study on Extended-Spectrum Beta-Lactamase-E. coli in Sentinel Mallard Ducks in an Important Baltic Stop-Over Site for Migratory Ducks in Germany" Microorganisms 10, no. 10: 1968. https://doi.org/10.3390/microorganisms10101968