Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation
AbstractBinary Ti alloys containing Fe, Mo, V and Zr were micro-arc oxidized and hydrothermally treated to obtain micro- and nano-porous layers. This study aimed to investigate cell differentiation on micro and micro/nanoporous oxide layers of Ti alloys. The properties of the porous layer formed on Ti alloys were characterized by X-ray diffraction pattern, microstructural and elemental analyses and inductively coupled plasma mass spectrometry (ICP-MS) method. The MTT assay, total protein production and alkaline phosphatase (ALPase) activity were evaluated using human osteoblast-like cells (MG-63). Microporous structures of micro-arc oxidized Ti alloys were changed to micro/nanoporous surfaces after hydrothermal treatment. Micro/nanoporous surfaces consisted of acicular TiO2 nanoparticles and micron-sized hydroxyapatite particles. From ICP and MTT tests, the Mo and V ions released from porous oxide layers were positive for cell viability, while the released Fe ions were negative for cell viability. Although the micro/nanoporous surfaces led to a lower total protein content than the polished and microporous Ti surfaces after cell incubation for 7 days, they caused higher ALPase activities after 7 days and 14 days of incubation except for V-containing microporous surfaces. The micro/nanoporous surfaces of Ti alloys were more efficient in inducing MG-63 cell differentiation. View Full-Text
Share & Cite This Article
Han, O.-S.; Hwang, M.-J.; Song, Y.-H.; Song, H.-J.; Park, Y.-J. Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation. Metals 2016, 6, 150.
Han O-S, Hwang M-J, Song Y-H, Song H-J, Park Y-J. Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation. Metals. 2016; 6(7):150.Chicago/Turabian Style
Han, Ok-Sung; Hwang, Moon-Jin; Song, Yo-Han; Song, Ho-Jun; Park, Yeong-Joon. 2016. "Effects of Surface Structure and Chemical Composition of Binary Ti Alloys on Cell Differentiation." Metals 6, no. 7: 150.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.