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Diagnostics 2018, 8(2), 33; https://doi.org/10.3390/diagnostics8020033

Angiogenesis PET Tracer Uptake (68Ga-NODAGA-E[(cRGDyK)]2) in Induced Myocardial Infarction and Stromal Cell Treatment in Minipigs

1
Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Department of Biomedical Sciences, Rigshospitalet and University of Copenhagen, Blegdamsvej 9, 2100 Copenhagen, Denmark
2
Cardiology Stem Cell Centre, Department of Cardiology, The Heart Centre, Rigshospitalet, University of Copenhagen, 1165 Copenhagen, Denmark
3
Avram and Stella Goldstein-Goren Department of Biotechnology Engineering and Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel
4
Department of Experimental Medicine, University of Copenhagen, 1165 Copenhagen, Denmark
5
Department of Cardiothoracic Surgery, The Heart Centre, Rigshospitalet, University of Copenhagen, 1165 Copenhagen, Denmark
6
Clinical Biochemistry, Copenhagen University Hospital, Rigshospitalet, 2100 Copenhagen, Denmark
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 April 2018 / Revised: 4 May 2018 / Accepted: 14 May 2018 / Published: 16 May 2018
(This article belongs to the Section Medical Imaging)
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Abstract

Angiogenesis is considered integral to the reparative process after ischemic injury. The αvβ3 integrin is a critical modulator of angiogenesis and highly expressed in activated endothelial cells. 68Ga-NODAGA-E[(cRGDyK)]2 (RGD) is a positron-emission-tomography (PET) ligand targeted towards αvβ3 integrin. The aim was to present data for the uptake of RGD and correlate it with histology and to further illustrate the differences in angiogenesis due to porcine adipose-derived mesenchymal stromal cell (pASC) or saline treatment in minipigs after induction of myocardial infarction (MI). Three minipigs were treated with direct intra-myocardial injection of pASCs and two minipigs with saline. MI was confirmed by 82Rubidium (82Rb) dipyridamole stress PET. Mean Standardized Uptake Values (SUVmean) of RGD were higher in the infarct compared to non-infarct area one week and one month after MI in both pASC-treated (SUVmean: 1.23 vs. 0.88 and 1.02 vs. 0.86, p < 0.05 for both) and non-pASC-treated minipigs (SUVmean: 1.44 vs. 1.07 and 1.26 vs. 1.04, p < 0.05 for both). However, there was no difference in RGD uptake, ejection fractions, coronary flow reserves or capillary density in histology between the two groups. In summary, indications of angiogenesis were present in the infarcted myocardium. However, no differences between pASC-treated and non-pASC-treated minipigs could be demonstrated. View Full-Text
Keywords: myocardial perfusion; RGD; rubidium; cardiac positron-emission-tomography; angiogenesis; mesenchymal stromal cells; hydrogel; stem cell; myocardial infarction myocardial perfusion; RGD; rubidium; cardiac positron-emission-tomography; angiogenesis; mesenchymal stromal cells; hydrogel; stem cell; myocardial infarction
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Rasmussen, T.; Follin, B.; Kastrup, J.; Brandt-Larsen, M.; Madsen, J.; Emil Christensen, T.; Juhl, M.; Cohen, S.; Pharao Hammelev, K.; Holdflod Møller, C.; Peter Goetze, J.; Hasbak, P.; Kjær, A. Angiogenesis PET Tracer Uptake (68Ga-NODAGA-E[(cRGDyK)]2) in Induced Myocardial Infarction and Stromal Cell Treatment in Minipigs. Diagnostics 2018, 8, 33.

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