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Diagnostics 2017, 7(3), 51; doi:10.3390/diagnostics7030051

Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities

1
School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia
2
Life Sciences, Macfarlane Burnet Institute, Melbourne 3004, Australia
3
Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang 11800, Malaysia
*
Author to whom correspondence should be addressed.
Received: 10 August 2017 / Revised: 29 August 2017 / Accepted: 31 August 2017 / Published: 7 September 2017
(This article belongs to the Special Issue Novel Point-of-Care Technologies in Diagnostics)
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Abstract

Lateral flow assays (LFAs) are the mainstay of rapid point-of-care diagnostics, with the potential to enable early case management and transform the epidemiology of infectious disease. However, most LFAs only detect single biomarkers. Recognizing the complex nature of human disease, overlapping symptoms and states of co-infections, there is increasing demand for multiplexed systems that can detect multiple biomarkers simultaneously. Due to innate limitations in the design of traditional membrane-based LFAs, multiplexing is arguably limited to a small number of biomarkers. Here, we summarize the need for multiplexed LFA, key technical and operational challenges for multiplexing, inherent in the design and production of multiplexed LFAs, as well as emerging enabling technologies that may be able to address these challenges. We further identify important areas for research in efforts towards developing multiplexed LFAs for more impactful diagnosis of infectious diseases. View Full-Text
Keywords: lateral flow assay; point-of-care; multiplex; diagnostic test; infectious disease; resource-limited settings lateral flow assay; point-of-care; multiplex; diagnostic test; infectious disease; resource-limited settings
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mohd Hanafiah, K.; Arifin, N.; Bustami, Y.; Noordin, R.; Garcia, M.; Anderson, D. Development of Multiplexed Infectious Disease Lateral Flow Assays: Challenges and Opportunities. Diagnostics 2017, 7, 51.

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