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Article

Calcification and Diagenesis of Bacterial Colonies

by
Ninon Robin
1,
Sylvain Bernard
2,*,
Jennyfer Miot
2,
Marie-Madeleine Blanc-Valleron
1,
Sylvain Charbonnier
1 and
Gilles Petit
1
1
CR2P, Centre de Recherche sur la Paléobiodiversité et les Paléoenvironnements, UMR 7207, CNRS, MNHN, UPMC Univ. Paris 06, Paris 75005, France
2
IMPMC, Institut de Minéralogie, de Physique des Matériaux et de Cosmochimie, UMR 7590, Sorbonne Universités, CNRS, MNHN, UPMC Univ. Paris 06, IRD, Paris 75005, France
*
Author to whom correspondence should be addressed.
Minerals 2015, 5(3), 488-506; https://doi.org/10.3390/min5030488
Submission received: 15 May 2015 / Revised: 18 June 2015 / Accepted: 15 July 2015 / Published: 22 July 2015
(This article belongs to the Special Issue Biomineralization: Towards a Unification of Concepts in Chemistry, Physics, Earth Sciences and Biology)
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Abstract

:
Evidencing ancient interspecific associations in the fossil record may be challenging, particularly when bacterial organisms have most likely been degraded during diagenesis. Yet, documenting ancient interspecific associations may provide valuable insights into paleoenvironmental conditions and paleocommunities. Here, we report the multiscale characterization of contemporary and fossilized calcifying bacterial colonies found on contemporary shrimps from Mexico (La Paz Bay) and on 160-Ma old fossilized decapods (shrimps) from the Lagerstätte of La Voulte-sur-Rhône (France), respectively. We document the fine scale morphology, the inorganic composition and the organic signatures of both the contemporary and fossilized structures formed by these bacterial colonies using a combination of electron microscopies and synchrotron-based scanning transmission X-ray microscopy. In addition to discussing the mechanisms of carbonate precipitation by such bacterial colonies, the present study illustrates the degradation of bacterial remains occurring during diagenesis.

1. Introduction

Epibiosis, the association between two species in which one (the epibiont) spends a portion of its life attached to the surface of the second one (the basibiont) [1], has likely involved a broad diversity of taxa over geological time as evidenced by a number of studies having reported fossilized symbiotic organisms [2,3,4,5,6,7,8,9,10,11,12]. Yet, identifying such ancient interspecific associations in the fossil record may be challenging in the case of epibiotic micro-organisms, notably because of their poor resistance to diagenetic degradation. For instance, only some rare fossilized examples of epibiosis have been reported within Precambrian rocks such as the Gunflint Formation [13]. Nevertheless, documenting such interspecific associations over geological timescales may provide new insights into processes and mechanisms of evolution as well as into paleoenvironments and paleocommunities.
Although a number of studies have reported the (partial) preservation of resistant organic macromolecules such as sporopollenin or chitin [14,15,16,17], identification of remains of micro-organisms within fossilized structures remains complicated as microbial-like morphologies may also be produced through abiotic processes [18,19]. Adding to controversies, bio-alteration and burial-induced processes inevitably alter the original biochemical signatures of organic molecules [20,21]. As a result, the general paleobiological perception has long been that burial and diagenetic processes are detrimental to the preservation of biosignatures. Yet, metamorphic rocks may retain, to some extent, morphologically and geochemically recognizable traces of life [14,22,23,24,25].
Biomineralized micro-organisms appear more resistant to diagenesis than non-biomineralized ones [26,27,28,29]. For instance, biologically mediated calcification of bacteria, which is involved in the formation of stromatolites or microbialites [30,31,32], has been described as a key process contributing to their fossilization [27,29,30,33]. The propensity of biogenic organo-mineral assemblages to overcome degradation during diagenesis might be dependent upon a number of parameters, including the paleoenvironmental and diagenetic conditions. Undoubtedly, the original nature of the minerals, their mode of formation (biomineralization vs. abiotic precipitation) as well as their structural and textural relationships with the organic components play a crucial role. Comparative study of contemporary and fossilized biomineralized microbial structures may allow to better constrain the respective influence of all these parameters on the diagenetic evolution of biogenic organo-mineral assemblages.
Here, we report the multiscale characterization of organo-mineral structures observed on the carapace of contemporary decapods (shrimps) from La Paz Bay (Mexico) as well as on fossilized counterparts from the Callovian Lagerstätte of La Voulte-sur-Rhône, France [34]. Of note, a number of studies have reported the presence of epibiotic micro-organisms, such as diatoms, cliliates and bacteria, on extant decapods [10,35,36,37,38]. Here, the use of advanced analytical techniques including X-ray diffraction (XRD), scanning and transmission electron microscopies (SEM & TEM) and scanning transmission X-ray microscopy (STXM) allow documenting submicrometer-scale chemical and structural features within the contemporary specimens, identifying these structures as epibiotic calcifying bacterial colonies and discussing the mechanisms of carbonate precipitation by these micro-organisms. In addition, the comparison with fossilized counterparts from La Voulte-sur-Rhone allows discussing the morphological and geochemical evolution of such objects during burial and diagenesis.

2. Material and Methods

2.1. Investigated Specimens

The investigated contemporary calcifying bacterial colonies have been collected on the cuticle of extant decapods (shrimps) belonging to the species Farfantepenaeus brevirostris (Kingsley, 1878, MNHN.IU. Na8524) (Figure 1). These shrimps and their epibiotic bacterial colonies have been caught alive in the La Paz Bay (Mexico) and preserved, since their capture, in 70% alcohol in order to maintain their structural and chemical integrity. Epibiotic organo-mineral structures have been detected on the whole carapace (pleon, carapace and telson) of most of the 20 sampled specimens, covering up to 10% of the whole animal (Figure 1) [11].
The investigated fossilized counterparts have been observed on the carapace of two fossil decapods (Archeosolenocera straeleni Carriol and Riou, 1991, UMPC-150 and Aeger brevirostris Van Straelen, 1923, MNHN.F.R61863) morphologically preserved in three dimensions within carbonate concretions from the Callovian Lagerstätte of La Voulte-sur-Rhône (France) (Figure 1) [34]. Although a smaller number of fossilized epibiotic structures have been observed on these fossilized shrimps compared to their modern counterparts, they cover about 5% of their surface (Figure 1). Fossilization of these shrimps occurred rapidly [39], through a quite common mineralogical diagenetic sequence (apatite, calcite, gypsum, pyrite, chalcopyrite and galena), indicating quite low diagenetic pressure and temperature conditions.
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Figure 1. Images of the decapod materials hosting the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Photograph and schematic representation of a contemporary specimen of Farfantepenaeus brevirostris (Kingsley, 1878, MNHN.IU. Na8524), from La Paz Bay, Mexico. (b) Photograph and schematic representation of a fossilized specimen of Aeger brevirostris Van Straelen, 1923 (MNHN.F.R61863) from the Callovian of La Voulte-sur Rhône. Blue squares indicate the location of epibiotic calcifying bacterial colonies.
Figure 1. Images of the decapod materials hosting the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Photograph and schematic representation of a contemporary specimen of Farfantepenaeus brevirostris (Kingsley, 1878, MNHN.IU. Na8524), from La Paz Bay, Mexico. (b) Photograph and schematic representation of a fossilized specimen of Aeger brevirostris Van Straelen, 1923 (MNHN.F.R61863) from the Callovian of La Voulte-sur Rhône. Blue squares indicate the location of epibiotic calcifying bacterial colonies.
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2.2. X-Ray Diffraction

The mineralogy of the investigated recent epibionts has been determined by X-ray diffraction (XRD) using the Bruker D2 PHASER operating at the MNHN (Paris, France) with a Lynxeye detector (opening: 5.821° 2Theta) and a Ni-filtered Cu Kα radiation (30 kV, 10 mA) with a 0.02° step width. Fresh cuticles and cuticles covered by epibionts have been crushed in an agate mortar and mounted as thin smears on zero-background Si plates.

2.3. Scanning Electron Microscopy (SEM)

Both extant and fossil specimens have been characterized using the Zeiss Supra 55 Scanning Electron Microscope (SEM) operating at the IMPMC (Paris, France). Secondary electron images have been collected at 5 kV accelerating voltage and a working distance of 2.5 mm. Elemental compositions have been semi-quantitatively determined by energy dispersive X-ray spectrometry (EDXS) using a Bruker EDS QUANTAX detector (Bruker Corporation, Houston, TX, USA) and the software ESPRIT at 15 kV accelerating voltage and a working distance of 7.5 mm.

2.4. Focused Ion Beam (FIB) Milling

Focused ion beam (FIB) milling has been performed at low Ga-ion currents using the FEI STRATA DB 235 FIB system operating at the IEMN (Lille, France) to prepare electron and soft X-ray transparent ~15 μm × 5 μm × 80 nm cross-sections [40]. This extraction procedure maintains textural integrity, even in the case of loosely consolidated materials [41,42], and prevents shrinkage and deformation of microscale to nanoscale pores, even in the case of highly sensitive materials [43,44]. Milling at low Ga-ion currents has allowed preventing common artifacts like local gallium implantation, mixing of components, creation of vacancies or interstitials, creation of amorphous layers, local composition changes or redeposition of the sputtered material on the sample surface [41,42,45,46,47]. In addition, milling at low Ga-ion currents prevents significant changes in the speciation of complex carbon-based polymer [15,48].

2.5. Transmission Electron Microscopy (TEM)

Transmission electron microscopy (TEM) analyses of FIB section were performed with a 200 kV JEOL 2100 field emission gun (FEG) microscope (JEOL Ltd, Tokyo, Japan) operating at the IMPMC (Paris, France). Scanning transmission electron microscopy imaging was performed in high-angle annular dark field (HAADF) mode.

2.6. Scanning Transmission X-Ray Microscopy (STXM) and X-Ray Absorption Near Edge Structure (XANES) Spectroscopy

X-ray absorption near edge structure (XANES) measurements of the present study were done using the Scanning transmission X-ray microscope (STXM) located on beamline 5.3.2.2 (STXM Polymer beamline) at the Advanced Light Source (ALS) [49]. Beamline 5.3.2.2. (ALS) uses soft X-rays (250–800 eV) generated via a bending magnet while the electron current in the storage ring is held constant in top-off mode at 500 mA at a storage ring energy of 1.9 GeV. The microscope chamber was evacuated to 100 mTorr after sample insertion and back-filled with He. Energy calibration was accomplished using the well-resolved 3p Rydberg peak at 294.96 eV of gaseous CO2 for the C K-edge. Alignment of images of stacks and extraction of XANES spectra were done using the aXis2000 software (Ver 2.1n). Spectral peak positions, intensities and widths were determined using the Athena software package [50]. The XANES spectra shown in the present contribution correspond to homogeneous organic-rich areas of several hundreds of nanometers. Although radiation damage per unit of analytical information has been shown to be typically 100–1000 times lower in STXM-based XANES spectroscopy than in TEM-based electron energy loss spectroscopy (EELS) [51,52,53,54], the C-XANES data shown here have been collected following the procedures for X-ray microscopy studies of radiation sensitive samples [55].

3. Results

3.1. Contemporary Epibiotic Organo-Mineral Structures

3.1.1. Morphology

The investigated contemporary epibiotic organo-mineral structures appear as rigid, flat and transparent structures fixed on the shrimp cuticle. They exhibit discoid, lobed, or rhizoid morphologies (Figure 2). They measure 50–800 μm in diameter and may be either found isolated or adjoined (Figure 2). The surface of these epibiotic structures is either quite smooth or highly ornamented (Figure 3). Some of them exhibit a smooth rosette at their center and highly ornamented areas in the periphery (Figure 3). High magnification observations reveal that these ornamentations consist in the agglomeration of globular structures measuring ~1.5 μm in diameter (Figure 3). Observations of transversal sections of these globular structures reveal their stick-like morphologies in three-dimensions that outcrop at the surface of the bacterial colony in the form of globules (Figure 3). The surface of these sticks appears covered by layers of a smooth and lumpy film resembling a microbial mat-like structure (Figure 3). Both the size and morphology of these stick-like forms evoke bacteria. In a similar perspective, the sizes and morphologies of the discoid, lobed and rhizoid structures formed by the assemblage of these sticks are consistent with bacterial colonies at different stages of growth [56,57]. Observations of cross-sections reveal that these epibiotic structures have a mean thickness of about 15 μm with a thicker central part (Figure 4).
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Figure 2. Morphologies of the investigated specimens of epibiotic calcifying bacterial colonies: schematic representations (a), photomicrographs of contemporary (b) and fossilized (c) specimens illustrating the discoid, the lobed and the rhizoid radial morphologies and showing adjoined colonies.
Figure 2. Morphologies of the investigated specimens of epibiotic calcifying bacterial colonies: schematic representations (a), photomicrographs of contemporary (b) and fossilized (c) specimens illustrating the discoid, the lobed and the rhizoid radial morphologies and showing adjoined colonies.
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Figure 3. Fine-scale morphologies of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) schematic representations, (b) SEM images (secondary electrons) of contemporary specimens and (c) SEM images (secondary electrons) of fossilized specimens at different magnifications (Cut = decapod cuticle, Or = ornamentations). (d) Schematic representation and SEM image (secondary electrons) of an incised surface of a contemporary epibiotic bacterial colony exhibiting stick-like structures outcropping at the surface in the form of globules.
Figure 3. Fine-scale morphologies of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) schematic representations, (b) SEM images (secondary electrons) of contemporary specimens and (c) SEM images (secondary electrons) of fossilized specimens at different magnifications (Cut = decapod cuticle, Or = ornamentations). (d) Schematic representation and SEM image (secondary electrons) of an incised surface of a contemporary epibiotic bacterial colony exhibiting stick-like structures outcropping at the surface in the form of globules.
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Figure 4. Thicknesses of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) SEM image (secondary electrons) of a section of a contemporary specimen. (b) SEM image (secondary electrons) of a section of a fossilized specimen.
Figure 4. Thicknesses of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) SEM image (secondary electrons) of a section of a contemporary specimen. (b) SEM image (secondary electrons) of a section of a fossilized specimen.
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3.1.2. Composition

The iridescence of the surface of the investigated contemporary epibiotic organo-mineral structures when illuminated with polarized light indicates a crystalline nature (Figure 5). XRD patterns of crushed samples reveal that these objects are made of calcite while epibiont-free cuticles display a typical alpha-chitinous XRD pattern (Figure 5). EDXS analyses confirm the calcitic composition of the investigated epibiotic structures and evidence low concentrations of iron and magnesium. Significant amounts of carbon and nitrogen are systematically observed in association with this low Mg-calcite (Figure 5).
TEM and STXM images of FIB foils reveal an alternation of mineralized stick-like structures with non-mineralized filaments of a few hundreds of nanometers (Figure 6). The C-XANES spectrum of the stick-like structures exhibits a main peak at 290.3 eV attributed to 1s → π* electronic transitions of carbon in carbonate groups (CO3) (Figure 6) [58] and a second absorption peak at 288.6 eV, generally attributed to carboxylic functional groups [59]. Such C-XANES spectra have been previously attributed to carbonates closely associated with organics [60,61,62]. These stick-like low Mg-carbonates closely associated with organics are consistent with calcified microbial remains [63,64,65].
The non-mineralized filaments located in between the carbonate stick-like structures exhibit a C-XANES spectrum with a main absorption peak at 288.2 eV, attributed to 1s → π* electronic transitions of carbon in amide groups, a peak at 285.2 eV, attributed to electronic transitions of carbon in aromatic or olefinic carbon groups, and two additional peaks at 287.3 and 289.4 eV, attributed to 1s → π* or 1s → 3p/σ* electronic transitions of hydroxylated- or ether-linked carbon species, respectively (Figure 6) [59]. The carbon functional groups responsible for the presence of these peaks may enter the composition of proteins (aromatic and amide groups), lipids (aliphatic groups) and polysaccharides (carboxylic groups). The spectrum of these filaments is thus typical of bacterial organic matter [59,60,61,62,66]. This bacterial origin is confirmed by XANES analyses at the N K-edge. The N-XANES spectra of these organics exhibit two sharp peaks at 399.8 and 401.2 eV, attributed to electronic transitions of nitrile (C≡N) and amidyl groups (CO-NHx), respectively, and a shoulder at 399 eV, likely corresponding to amine groups (Figure 6) [67,68,69].
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Figure 5. Inorganic composition of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Photomicrograph of iridescent contemporary specimens of epibiotic calcifying bacterial colonies. (b) XRD patterns of contemporary epibiotic calcifying bacterial colonies and of a piece of the host decapod cuticle free of epibiotic bacterial colony. While the host cuticle exhibit an alpha-chitinous signal, contemporary epibiotic calcifying bacterial colonies exhibit peaks consistent with calcite planes. (c) EDXS signals of more or less mineralized contemporary and fossilized epibiotic calcifying bacterial colonies. All signals are consistent with Ca carbonates containing small amounts of Fe and Mg. Note that the organics can be identified from the presence of C and N in partially mineralized contemporary and fossilized epibiotic calcifying bacterial colonies.
Figure 5. Inorganic composition of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Photomicrograph of iridescent contemporary specimens of epibiotic calcifying bacterial colonies. (b) XRD patterns of contemporary epibiotic calcifying bacterial colonies and of a piece of the host decapod cuticle free of epibiotic bacterial colony. While the host cuticle exhibit an alpha-chitinous signal, contemporary epibiotic calcifying bacterial colonies exhibit peaks consistent with calcite planes. (c) EDXS signals of more or less mineralized contemporary and fossilized epibiotic calcifying bacterial colonies. All signals are consistent with Ca carbonates containing small amounts of Fe and Mg. Note that the organics can be identified from the presence of C and N in partially mineralized contemporary and fossilized epibiotic calcifying bacterial colonies.
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Figure 6. Organic composition of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Top: TEM image of an ultrathin FIB foil of sample extracted from a contemporary epibiotic calcifying bacterial colony (red squares indicate the location of TEM images shown in (b)). Bottom: STXM compositional map of the ultrathin foil showing the spatial distribution of carbonates (blue) and organics (yellow). (b) TEM images of areas of the ultrathin FIB foil shown in (a). Organic filaments appear in dark. (c) Top: C-XANES spectra of organics and carbonates composing the ultrathin FIB foil shown in (a) (peaks at 285.1, 286.7, 288.2, 288.6, 289.5 and 290.3 eV are attributed to electronic transitions of carbon involved in aromatic of olefinic, phenolic, amidyl, carboxylic, hydroxylated and carbonate groups, respectively). Bottom: XANES spectrum of the epibiotic bacterial organic matter collected over the carbon and nitrogen absorption edge showing its N-rich nature (atomic N/C = 0.2).
Figure 6. Organic composition of the investigated contemporary and fossilized epibiotic calcifying bacterial colonies. (a) Top: TEM image of an ultrathin FIB foil of sample extracted from a contemporary epibiotic calcifying bacterial colony (red squares indicate the location of TEM images shown in (b)). Bottom: STXM compositional map of the ultrathin foil showing the spatial distribution of carbonates (blue) and organics (yellow). (b) TEM images of areas of the ultrathin FIB foil shown in (a). Organic filaments appear in dark. (c) Top: C-XANES spectra of organics and carbonates composing the ultrathin FIB foil shown in (a) (peaks at 285.1, 286.7, 288.2, 288.6, 289.5 and 290.3 eV are attributed to electronic transitions of carbon involved in aromatic of olefinic, phenolic, amidyl, carboxylic, hydroxylated and carbonate groups, respectively). Bottom: XANES spectrum of the epibiotic bacterial organic matter collected over the carbon and nitrogen absorption edge showing its N-rich nature (atomic N/C = 0.2).
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3.2. Fossilized Epibiotic Structures

The investigated fossilized epibiotic structures appear as rigid and flat structures fixed on the cuticle of fossilized decapods (shrimps) from the Callovian Lagerstätte of La Voulte-sur-Rhône (France). Similarly to the contemporary epibiotic structures investigated in the present study, these fossilized structures exhibit discoid, lobed, or rhizoid morphologies (Figure 2). They measure 50–500 μm in diameter (with a few of displaying a diameter up to 600 μm). Most of them are isolated but some may be found contiguous to each other (Figure 2). Although the surface of these epibiotic structures appears structure-free by optical microscopy (Figure 3), high magnification SEM observations reveal fine-scale ornamentations consisting in the agglomeration of globular structures of 1–2 μm in diameter (Figure 3). Observations of cross-sections reveal a total thickness of ~15 μm (Figure 4). As shown by EDXS analyses, the investigated epibiotic structures have a calcitic composition with low concentrations of iron and magnesium (Figure 5). No organic matter has been detected in association with these fossilized calcified epibiotic structures using either EDXS or XANES spectroscopy.

4. Discussion

Here, we report the multiscale characterization of contemporary organo-mineral epibiotic structures that we identify as bacterial colonies based on their morphological and geochemical signatures. As the investigated contemporary and fossilized epibiotic structures share numerous similarities down to the submicrometer scale, the fossilized epibiotic structures are interpreted as fossilized calcifying epibiotic bacterial colonies. Of note, a palaeoecological complementary study has revealed the probable ectoparasitic habit of these bacterial colonies [11]. This symbiosis close to parasitism has thus existed for at least 160 myr. Here, we first discuss the possible biomineralization processes leading to the precipitation of low Mg calcite by these bacterial colonies. Then, based on the comparison between the investigated contemporary and fossilized specimens, we discuss the impact of diagenetic processes on the preservation/degradation of the biosignatures of such calcifying bacterial colonies.

4.1. Biomineralization of Calcified Epibiotic Bacterial Colonies

The highly structured sub-micrometric association of calcite with organic molecules dominated by proteic moieties exhibited by the contemporary bacterial colonies suggests a formation resulting from biomineralization processes [60,70]. Two main pathways of bacteria-induced/controlled carbonate precipitation have been reported in modern environments and laboratory cultures [64,71,72,73,74].
(1) The formation of miscellaneous crystalline carbonates (e.g., aragonite, Mg-rich or Mg-low calcite, dolomite) may result from extracellular precipitation [72,75,76,77,78]. In this case, negatively charged groups exposed at the surface of the cell and/or of exopolymeric substances (EPS) act as mineral nucleation sites for carbonate precipitation, so that organic matter is intimately associated with carbonate minerals. This process is dependent upon the microenvironmental chemical conditions (Ca2+ and dissolved inorganic carbon concentrations) that can be controlled by biological activity from different metabolisms such as sulfate-reduction, oxygenic or anoxygenic photosynthesis [63,79,80,81,82].
(2) Alternatively, biomineralization may occur intracellularly. Intracellular carbonate precipitation, as recently evidenced for multiple strains of cyanobacteria [73,83], may lead to the accumulation of calcium carbonates within bacterial cells. Whereas gas vesicles function as flotation devices in planktonic prokaryotes [84], the precipitation of intracellular carbonates has been proposed to potentially serve as ballasts [73], thus facilitating benthic (or epibiotic) modes of life. Additionally, such carbonate precipitation has been proposed to possibly act as a pH buffering system [73] that may facilitate carbon concentrating mechanisms and thus improve bacterial colony growth rate.
Here, we evidence the presence of ~1 μm wide stick-like carbonate structures exhibiting an absorption peak at 288.6 eV in their C-XANES spectra. This peak could be attributed to the presence of EPS [85]. These minerals may thus result from extracellular precipitation of amorphous carbonates that may preferentially occur onto EPS molecules. The vertical orientation of the carbonate sticks may be related to that of the calcifying bacteria anchored at the surface of the decapod cuticle, as it has already been observed for filamentous cyanobacteria in stromatolites [74]. In these formations, heterotrophic degradation of cyanobacterial EPS is generally invoked to explain the progressive replacement of amorphous carbonates by calcite, leading to a porous microstructure composed of bacterial filaments and carbonates [74].
Alternatively, as the carbonate stick-like structures described here exhibit sizes and morphologies comparable to cyanobacteria, they might be interpreted as resulting from intracellular bacterial calcification. In this scenario, the organic filaments observed around these stick-like carbonates might constitute remains of bacterial membranes. The precipitation of carbonates within the bacterial cells may have first served to facilitate the long-term fixation of bacterial colonies on the decapod cuticles. Although these carbonates might have first been small amorphous spheres within the bacteria cells, they may have merged and evolved towards crystalline calcite after the death of bacteria [78].

4.2. Fossilization of Calcifying Bacterial Colonies

Preservation of chemical and/or morphological bacterial signatures in fossils is promoted by several factors in addition to limited heterotrophic processes (i.e., biodegradation of organic matter) [33]. For instance, carbonate and phosphate biomineralization has been shown to efficiently preserve molecular signatures of biogenic organic molecules [28,29]. In addition, the location of mineral precipitation with respect to the bacterial ultrastructures has also been shown to influence the extent of biosignature preservation. For instance, periplasm encrustation has been described as favoring bacterial morphology preservation [26,61,86]. Specific crystallographic orientation (perpendicular to cell walls) of crystalline phases precipitated within bacterial periplasm [61,86,87], at the cell surface or in association with cyanobacterial sheaths [32] may also promote the preservation of specific biosignatures upon fossilization and diagenetic processes. Last, trapping of organics within minerals upon diagenesis and even metamorphism has been shown to allow, to some extent, the preservation of geochemical biosignatures [14,24,25,29,88].
Here, the morphologies of the fossilized calcifying bacterial colonies appear very similar to the ones of the contemporary colonies, even down to the submicrometer scale. Such fine-scale morphological preservation likely results from the early mineralization of bacterial cells. Yet, no organic carbon has been detected in association with the fossil epibionts using EDXS or XANES spectroscopy. The bacterial organics originally constituting the fossilized calcifying epibiotic bacterial colonies have thus been completely degraded during fossilization and burial processes. Such loss of organic carbon upon fossilization suggests that carbonate biomineralization did occur neither within bacterial cell walls nor at the cell surface. This observation appears in favor of an intracellular process of carbonate biomineralization as it may not promote organic carbon preservation as previously suggested [73]. Thus, the absence of a “bacterial signature” within mineral phases may not always be inconsistent with a bacterial origin and may modify the general perception of a number of specimens that might be worth re-exploring for the search for traces of ancient life in rocks.

5. Concluding Remarks

The multiscale characterization reported here strongly suggests that the investigated contemporary and fossilized epibiotic structures observed on modern and fossilized decapod cuticles can be identified as modern and fossilized calcifying bacterial colonies, respectively. Yet, the exact calcifying process responsible for the formation of these biomineralized structures, either intracellular or extracellular, remains difficult to constrain. The fine-scale morphologies of the investigated fossilized calcifying bacterial colonies appear to have been exceptionally well preserved down to the micrometer scale, likely as the result of the early mineralization of bacterial cells. Yet, the original bacterial organics have been totally degraded during fossilization and burial. Such degradation upon fossilization, despite the rather low pressure and temperature diagenetic conditions experienced by these samples, may be ascribed to particularities of the biomineralization processes (absence of periplasmic or cell surface encrustation).

Acknowledgements

We gratefully acknowledge support from the ERC (project PaleoNanoLife-PI: F. Robert). Special thanks go to Simona Pestrea and Jean-Paul Saint Martin for their help with optical microscopy and fruitful discussions and special thanks go to David Troadec for the preparation of FIB sections. The SEM facility of the IMPMC is supported by Région Ile de France grant SESAME 2006 N°I-07-593/R, INSU-CNRS, INP-CNRS, UPMC–Paris 6, and by the Agence Nationale de la Recherche (ANR) grant No. ANR-07-BLAN-0124-01. Special thanks go to Imene Esteve for her expert support of the SEM at IMPMC. The TEM facility of the IMPMC is supported by Region Ile de France grant SESAME 2000 E 1435. Special thanks go to Jean-Michel Guigner for his expert support of the TEM at IMPMC. STXM-based XANES data were acquired at beamline 5.3.2.2 at the Advanced Light Source (ALS), which is supported by the Director of the Office of Science, Department of Energy, under Contract No. DE-AC02-05CH11231. Special thanks go to David Kilcoyne for his expert support of the STXM at the ALS. Comments from three reviewers have been very helpful in improving the present manuscript.

Author Contributions

N.R., S.B., J.M., S.C. and G.P. conceived and designed the experiments. S.C. and G.P. provided the samples. N.R., S.B., J.M. and M.M.B.V. performed the experiments. N.R., S.B. and J.M. interpreted the data and wrote the present article.

Conflicts of Interest

The authors declare no conflict of interest.

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Robin, N.; Bernard, S.; Miot, J.; Blanc-Valleron, M.-M.; Charbonnier, S.; Petit, G. Calcification and Diagenesis of Bacterial Colonies. Minerals 2015, 5, 488-506. https://doi.org/10.3390/min5030488

AMA Style

Robin N, Bernard S, Miot J, Blanc-Valleron M-M, Charbonnier S, Petit G. Calcification and Diagenesis of Bacterial Colonies. Minerals. 2015; 5(3):488-506. https://doi.org/10.3390/min5030488

Chicago/Turabian Style

Robin, Ninon, Sylvain Bernard, Jennyfer Miot, Marie-Madeleine Blanc-Valleron, Sylvain Charbonnier, and Gilles Petit. 2015. "Calcification and Diagenesis of Bacterial Colonies" Minerals 5, no. 3: 488-506. https://doi.org/10.3390/min5030488

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