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Minerals 2012, 2(1), 40-54; doi:10.3390/min2010040

Fits and Misfits in Organic Matrix Analyses: Case of the Soluble Matrices of the Nacreous Layer of Pinctada margaritifera (Mollusca)

UMR 8148 Interactions et Dynamique des Environnements de Surface, Bat. 504, Université Paris Sud, Orsay Cedex 91405, France
Author to whom correspondence should be addressed.
Received: 17 January 2012 / Revised: 16 February 2012 / Accepted: 17 February 2012 / Published: 27 February 2012
(This article belongs to the Special Issue Advances in Biominerals)
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Mollusk shells, especially the nacre, are of commercial interest as well as palaeoenvironmental proxies. They are also investigated as biomaterials for medical purposes and biomimetics. Although the mineralogy is well-known and unique (aragonite tablets), the organic components are various. However, determination of the precise composition of the soluble organic matrix (SOM) of the nacreous layer is difficult. Among the range of possible techniques, 1D electrophoresis and High-performance liquid chromatography (HPLC) have previously been applied separately to differentiate pI and molecular weights. To date, no clear correlation has been established between the two parameters obtained in such conditions. Here, we report the use of preparative electrophoresis, coupled with HPLC, to determine the molecular weights of the pI fractions. The results are compared with 2D gel electrophoresis. It is shown that both methods have drawbacks and advantages, and are not redundant. The complexity of the composition of the nacreous tablet shown by scanning electron microscope (SEM) and Atomic Force Microscope (AFM) observations is also evidenced by electrophoresis and HPLC. View Full-Text
Keywords: soluble organic matrix; mollusk shell; nacre; electrophoresis; HPLC soluble organic matrix; mollusk shell; nacre; electrophoresis; HPLC

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Dauphin, Y.; Nouet, J. Fits and Misfits in Organic Matrix Analyses: Case of the Soluble Matrices of the Nacreous Layer of Pinctada margaritifera (Mollusca). Minerals 2012, 2, 40-54.

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