Next Article in Journal
Spatiotemporal Interpolation of Rainfall by Combining BME Theory and Satellite Rainfall Estimates
Previous Article in Journal
Characteristics of Gravity Waves over an Antarctic Ice Sheet during an Austral Summer
Article Menu

Export Article

Open AccessArticle
Atmosphere 2015, 6(9), 1290-1306; doi:10.3390/atmos6091290

Effects of Stratospheric Conditions on the Viability, Metabolism and Proteome of Prokaryotic Cells

1
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
3
Department of Geology and Pedology, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
4
Department of Forest Management and Applied Geoinformatics, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Robert W. Talbot
Received: 29 July 2015 / Revised: 18 August 2015 / Accepted: 20 August 2015 / Published: 28 August 2015
View Full-Text   |   Download PDF [5884 KB, uploaded 28 August 2015]   |  

Abstract

The application of ultraviolet (UV) radiation to inhibit bacterial growth is based on the principle that the exposure of DNA to UV radiation results in the formation of cytotoxic lesions, leading to inactivation of microorganisms. Herein, we present the impacts of UV radiation on bacterial cultures’ properties from the biological, biochemical and molecular biological perspective. For experiments, commercial bacterial cultures (Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli and Salmonella typhimurium) and isolates from patients with bacterial infections (Proteus mirabilis and Pseudomonas aeruginosa) were employed. The above-mentioned strains were exposed to UV using a laboratory source and to stratospheric UV using a 3D printed probe carried by a stratospheric balloon. The length of flight was approximately two hours, and the probe was enriched by sensors for the external environment (temperature, pressure and relative humidity). After the landing, bacterial cultures were cultivated immediately. Experimental results showed a significant effect of UV radiation (both laboratory UV and UV from the stratosphere) on the growth, reproduction, behavior and structure of bacterial cultures. In all parts of the experiment, UV from the stratosphere showed stronger effects when compared to the effects of laboratory UV. The growth of bacteria was inhibited by more than 50% in all cases; moreover, in the case of P. aeruginosa, the growth was even totally inhibited. Due to the effect of UV radiation, an increased susceptibility of bacterial strains to environmental influences was also observed. By using commercial tests for biochemical markers of Gram-positive and Gram-negative strains, significant disparities in exposed and non-exposed strains were found. Protein patterns obtained using MALDI-TOF mass spectrometry revealed that UV exposure is able to affect the proteins’ expression, leading to their downregulation, observed as the disappearance of their peaks from the mass spectrum. View Full-Text
Keywords: stratosphere; ultraviolet; radiation; prokaryotes; 3D chips; proteomics stratosphere; ultraviolet; radiation; prokaryotes; 3D chips; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chudobova, D.; Cihalova, K.; Jelinkova, P.; Zitka, J.; Nejdl, L.; Guran, R.; Klimanek, M.; Adam, V.; Kizek, R. Effects of Stratospheric Conditions on the Viability, Metabolism and Proteome of Prokaryotic Cells. Atmosphere 2015, 6, 1290-1306.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Atmosphere EISSN 2073-4433 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top