Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Antibodies and Dyes
2.3. Cell Culture
2.4. Preparing Cells for Tumour Implantation
2.5. Tumor Implantation
2.6. Surgical Preparation of Subcutaneous Tumours
2.7. Surgical Preparation of Intramuscular Tumors
2.8. IVM Imaging
2.9. Cell Tracking Analysis
3. Results
3.1. Multimodal IVM Imaging of the TME
3.2. IVM of Leukocyte Behavior in Tumor Arterioles and Venules
3.3. IVM of Leukocyte Behavior in Tumor Microcirculation/Capillaries
3.4. Tracking Leukocytes in the Tumor Interstitium by IVM
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Turk, M.; Naumenko, V.; Mahoney, D.J.; Jenne, C.N. Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches. Cells 2018, 7, 69. https://doi.org/10.3390/cells7070069
Turk M, Naumenko V, Mahoney DJ, Jenne CN. Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches. Cells. 2018; 7(7):69. https://doi.org/10.3390/cells7070069
Chicago/Turabian StyleTurk, Madison, Victor Naumenko, Douglas J. Mahoney, and Craig N. Jenne. 2018. "Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches" Cells 7, no. 7: 69. https://doi.org/10.3390/cells7070069