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Cells 2018, 7(7), 69; https://doi.org/10.3390/cells7070069

Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches

1
,
1,2
,
1,3,4,5,†,* and 1,6,†,*
1
Department of Microbiology, Immunology and Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
2
National University of Science and Technology “MISIS”, Leninskiy Prospect 4, 119991 Moscow, Russia
3
Alberta Children’s Hospital Research Institute, Calgary, AB T2N 4N1, Canada
4
Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4N1, Canada
5
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
6
Department of Critical Care Medicine, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
Craig N. Jenne and Douglas J. Mahoney contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 May 2018 / Revised: 18 June 2018 / Accepted: 29 June 2018 / Published: 3 July 2018
(This article belongs to the Special Issue Innovative Methods to Monitor Single Live Cells)
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Abstract

Recent advances in imaging technology have made it possible to track cellular recruitment and behavior within the vasculature of living animals in real-time. Using approaches such as resonant scanning confocal and multiphoton intravital microscopy (IVM), we are now able to observe cells within the intact tumor microenvironment of a mouse. We are able to follow these cells for extended periods of time (hours) and can characterize how specific cell types (T cells, neutrophils, monocytes) interact with the tumor vasculature and cancer cells. This approach provides greater insight into specific cellular behaviors and cell–cell interactions than conventional techniques such as histology and flow cytometry. In this report, we describe the surgical preparation of animals to expose the tumor and both resonant scanning confocal and multiphoton imaging approaches used to track leukocyte recruitment, adhesion, and behavior within the tumor microenvironment. We present techniques for the measurement and quantification of leukocyte behavior within the bloodstream and tumor interstitium. The use of IVM to study leukocyte behavior within the tumor microenvironment provides key information not attainable with other approaches, that will help shape the development of better, more effective anticancer drugs and therapeutic approaches. View Full-Text
Keywords: intravital; cancer; vasculature; trafficking; leukocytes; imaging intravital; cancer; vasculature; trafficking; leukocytes; imaging
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Turk, M.; Naumenko, V.; Mahoney, D.J.; Jenne, C.N. Tracking Cell Recruitment and Behavior within the Tumor Microenvironment Using Advanced Intravital Imaging Approaches. Cells 2018, 7, 69.

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