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Cells 2018, 7(7), 66; https://doi.org/10.3390/cells7070066

The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells

1
Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA
2
Department of Cell Biology, Emory University, Atlanta, GA 30322, USA
These authors contributed equally.
*
Author to whom correspondence should be addressed.
Received: 10 March 2018 / Revised: 21 June 2018 / Accepted: 22 June 2018 / Published: 26 June 2018
(This article belongs to the Special Issue Cell Adhesion Molecules)
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Abstract

Cell-cell junctions are critical structures in a number of tissues for mechanically coupling cells together, cell-to-cell signaling, and establishing a barrier. In many tissues, desmosomes are an important component of cell-cell junctions. Loss or impairment of desmosomes presents with clinical phenotypes in the heart and skin as cardiac arrhythmias and skin blistering, respectively. Because heart and skin are tissues that are subject to large mechanical stresses, we hypothesized that desmosomes, similar to adherens junctions, would also experience significant tensile loading. To directly measure mechanical forces across desmosomes, we developed and validated a desmoglein-2 (DSG-2) force sensor, using the existing TSmod Förster resonance energy transfer (FRET) force biosensor. When expressed in human cardiomyocytes, the force sensor reported high tensile loading of DSG-2 during contraction. Additionally, when expressed in Madin-Darby canine kidney (MDCK) epithelial or epidermal (A431) monolayers, the sensor also reported tensile loading. Finally, we observed higher DSG-2 forces in 3D MDCK acini when compared to 2D monolayers. Taken together, our results show that desmosomes experience low levels of mechanical tension in resting cells, with significantly higher forces during active loading. View Full-Text
Keywords: desmosomes; mechanobiology; cell biophysics desmosomes; mechanobiology; cell biophysics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Baddam, S.R.; Arsenovic, P.T.; Narayanan, V.; Duggan, N.R.; Mayer, C.R.; Newman, S.T.; Abutaleb, D.A.; Mohan, A.; Kowalczyk, A.P.; Conway, D.E. The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells. Cells 2018, 7, 66.

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