Angiotensin II-Induced Cardiac Effects Are Modulated by Endocannabinoid-Mediated CB1 Receptor Activation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Langendorff Heart Preparation
2.3. Experimental Protocol
2.4. Immunohistochemistry
2.5. Immunofluorescent Visualization of CB1 Receptors in Rat Cardiac Tissue
2.6. Statistical Analysis and Interpretation
3. Results
3.1. CB1 Receptors in Rat Cardiac Tissue
3.2. The Effects of Ang II Infusion on Isolated Rat Hearts
3.3. Effects of Repetitive Ang II Infusion on Coronary Flow and Contractile Function
3.4. Effects of CB Receptor Agonists and CB1R Antagonist O2050 on Coronary Flow and Contractile Function
3.5. The Influence of CB1R Antagonist O2050 and DAGL Inhibitor Orlistat on Ang II Effects
4. Discussion
4.1. Effects of Endocannabinoids on Cardiac Function
4.2. Role of Angiotensin II-Induced Endocannabinoid Release in Cardiac Function
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Miklós, Z.; Wafa, D.; Nádasy, G.L.; Tóth, Z.E.; Besztercei, B.; Dörnyei, G.; Laska, Z.; Benyó, Z.; Ivanics, T.; Hunyady, L.; et al. Angiotensin II-Induced Cardiac Effects Are Modulated by Endocannabinoid-Mediated CB1 Receptor Activation. Cells 2021, 10, 724. https://doi.org/10.3390/cells10040724
Miklós Z, Wafa D, Nádasy GL, Tóth ZE, Besztercei B, Dörnyei G, Laska Z, Benyó Z, Ivanics T, Hunyady L, et al. Angiotensin II-Induced Cardiac Effects Are Modulated by Endocannabinoid-Mediated CB1 Receptor Activation. Cells. 2021; 10(4):724. https://doi.org/10.3390/cells10040724
Chicago/Turabian StyleMiklós, Zsuzsanna, Dina Wafa, György L. Nádasy, Zsuzsanna E. Tóth, Balázs Besztercei, Gabriella Dörnyei, Zsófia Laska, Zoltán Benyó, Tamás Ivanics, László Hunyady, and et al. 2021. "Angiotensin II-Induced Cardiac Effects Are Modulated by Endocannabinoid-Mediated CB1 Receptor Activation" Cells 10, no. 4: 724. https://doi.org/10.3390/cells10040724