Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of JCS1 and Its Acetylated Derivative
2.2.1. Preparation of JCS1
2.2.2. Preparation of Acetylated Derivative
2.3. Homogeneity and Molecular Weight
2.4. Monosaccharide Composition Analysis
2.5. Methylation Analysis
2.6. Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) Analysis
2.7. Partial Acid Hydrolysis
2.8. Bioactivity Test of Polysaccharide in PC-12 Cells
3. Results
3.1. Isolation, Purification, and Composition Analysis
3.2. IR and Specific Rotation Analysis
3.3. Linkage Type Analysis
3.4. Partial Acid Hydrolysis and Structure Characterization of Degraded Polysaccharide
3.5. NMR Results
3.6. YJCS1 Induces Neurite Extension of PC-12 Cells
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Methylated Sugars | Linkages | Molar Ratio % | |
---|---|---|---|
JCS1 | JCS10.1N * | ||
2,3,6-Me3-Glc | 1,4-Glcp | 34.2 | 17.8 |
2,3,4,6-Me4-Glc | T-Glcp | 2.6 | 2.6 |
2,3-Me2-Glc | 1,4,6-Glcp | 3.2 | 3.0 |
2,3,6-Me3-Man | 1,4-Manp | 2.2 | 2.3 |
2,3-Me2-Xyl | 1,4-Xylp | 1.6 | 0.6 |
2,3,5-Me3-Ara | T-Araf | 1.0 | _ |
Residues | 1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|---|
1,4-α-Glcp | H | 5.46 | 3.71 | 4.02 | 3.72 | 3.73 | 3.90 |
C | 100.83 | 72.75 | 74.53 | 78.02 | 72.45 | 61.57 | |
T-α-Glcp | H | 5.03 | 3.68 | 3.83 | 3.49 | 3.79 | 3.90 |
C | 99.80 | 71.94 | 74.06 | 69.84 | 72.76 | 61.65 | |
1,4,6-α-Glcp | H | 5.42 | 3.69 | 4.00 | 3.72 | 3.99 | 3.72/4.02 |
C | 101.07 | 71.46 | 73.50 | 78.02 | 74.93 | 70.40 | |
1,4-β-Manp | H | 4.55 | 3.36 | 3.85 | 3.72 | 3.84 | 3.75 |
C | 102.93 | 73.95 | 73.61 | 77.58 | 71.51 | 64.09 | |
1,4-α-Xylp | H | 5.14 | 3.68 | 3.71 | 3.72 | 3.72 | - |
C | 99.22 | 73.61 | 73.02 | 76.70 | 61.25 | - | |
T-α-Araf | H | 5.11 | 4.20 | 4.03 | 4.12 | 3.46 | - |
C | 109.08 | 82.57 | 76.66 | 83.56 | 64.07 | - | |
T-α-Araf | H | 5.24 | 4.26 | nd | nd | nd | - |
C | 107.44 | nd | nd | nd | nd | - |
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Jin, C.; Du, Z.; Lin, L.; Zhou, L.; Li, S.; Liu, Q.; Ding, K. Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells. Polymers 2017, 9, 399. https://doi.org/10.3390/polym9090399
Jin C, Du Z, Lin L, Zhou L, Li S, Liu Q, Ding K. Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells. Polymers. 2017; 9(9):399. https://doi.org/10.3390/polym9090399
Chicago/Turabian StyleJin, Can, Zhenyun Du, Liyan Lin, Lishuang Zhou, Saijuan Li, Qin Liu, and Kan Ding. 2017. "Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells" Polymers 9, no. 9: 399. https://doi.org/10.3390/polym9090399