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Polymers 2017, 9(9), 399; https://doi.org/10.3390/polym9090399

Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells

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1
School of Pharmacy, Zunyi Medical University, 201 Dalian Road, Zunyi 563003, China
2
Glycochemistry and Glycobiology Lab, Shangshai Institute of Materia Medica, Key Laboratory of Receptor Research, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China
3
University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Received: 20 July 2017 / Revised: 18 August 2017 / Accepted: 22 August 2017 / Published: 28 August 2017
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Abstract

A water-soluble polysaccharide (JCS1) was isolated from the stems of Dendrobium nobile Lindl. JCS1 was structurally characterized using a combination of chemical and spectral analysis, including methylation analysis, partial acid hydrolysis, Fourier-transform infrared (FTIR) spectroscopy, gas chromatography (GC), GC-mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. The molecular weight was estimated to be 2.3 × 104 Da using high-performance gel permeation chromatography (HPGPC). The sugar composition analysis indicated it was composed of glucose, mannose, xylose, and arabinose in a 40.2:2.3:1.7:1.0 molar ratio. The structure analysis showed that JCS1 was a mannoglucan with a backbone consisting of (1→4)-linked β-Manp and (1→4)-linked α-Glcp with branches at C-6 of (1→4)-linked α-Glcp residues. The branches were composed of T-α-Glcp, 1,4-α-Xylp, and T-α-Araf. In vitro bioactivity tests revealed that the acetylated derivative of JCS1, YJCS1, induced neuritogenesis of PC-12 cells. These results demonstrate that YJCS1 might be a promising bioactive polysaccharide for development as a drug candidate for the possible prevention and treatment of neurodegeneration diseases. View Full-Text
Keywords: mannoglucan; polysaccharide; Dendrobium nobile Lindl; neuritogenesis; neurodegeneration disease mannoglucan; polysaccharide; Dendrobium nobile Lindl; neuritogenesis; neurodegeneration disease
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Jin, C.; Du, Z.; Lin, L.; Zhou, L.; Li, S.; Liu, Q.; Ding, K. Structural Characterization of Mannoglucan from Dendrobium nobile Lindl and the Neuritogenesis-Induced Effect of Its Acetylated Derivative on PC-12 Cells. Polymers 2017, 9, 399.

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