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Polymers 2017, 9(8), 343; doi:10.3390/polym9080343

Tuning the Density of Poly(ethylene glycol) Chains to Control Mammalian Cell and Bacterial Attachment

Department of Chemistry and Biotechnology, School of Science, Faculty of Science, Engineering and Technology, Swinburne University of technology, Hawthorn, VIC 3122, Australia
ARC Training Centre for Biodevices, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia
Authors to whom correspondence should be addressed.
Received: 30 June 2017 / Revised: 1 August 2017 / Accepted: 2 August 2017 / Published: 5 August 2017
(This article belongs to the Special Issue Advance of Polymers Applied to Biomedical Applications: Biointerface)
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Surface modification of biomaterials with polymer chains has attracted great attention because of their ability to control biointerfacial interactions such as protein adsorption, cell attachment and bacterial biofilm formation. The aim of this study was to control the immobilisation of biomolecules on silicon wafers using poly(ethylene glycol)(PEG) chains by a “grafting to” technique. In particular, to control the polymer chain graft density in order to capture proteins and preserve their activity in cell culture as well as find the optimal density that would totally prevent bacterial attachment. The PEG graft density was varied by changing the polymer solubility using an increasing salt concentration. The silicon substrates were initially modified with aminopropyl-triethoxysilane (APTES), where the surface density of amine groups was optimised using different concentrations. The results showed under specific conditions, the PEG density was highest with grafting under “cloud point” conditions. The modified surfaces were characterised with X-ray photoelectron spectroscopy (XPS), ellipsometry, atomic force microscopy (AFM) and water contact angle measurements. In addition, all modified surfaces were tested with protein solutions and in cell (mesenchymal stem cells and MG63 osteoblast-like cells) and bacterial (Pseudomonas aeruginosa) attachment assays. Overall, the lowest protein adsorption was observed on the highest polymer graft density, bacterial adhesion was very low on all modified surfaces, and it can be seen that the attachment of mammalian cells gradually increased as the PEG grafting density decreased, reaching the maximum attachment at medium PEG densities. The results demonstrate that, at certain PEG surface coverages, mammalian cell attachment can be tuned with the potential to optimise their behaviour with controlled serum protein adsorption. View Full-Text
Keywords: PEG; surface modification; protein adsorption; cell attachment; biofilm formation PEG; surface modification; protein adsorption; cell attachment; biofilm formation

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Al-Ani, A.; Pingle, H.; P Reynolds, N.; Wang, P.-Y.; Kingshott, P. Tuning the Density of Poly(ethylene glycol) Chains to Control Mammalian Cell and Bacterial Attachment. Polymers 2017, 9, 343.

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