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Polymers 2017, 9(11), 576; https://doi.org/10.3390/polym9110576

A Cationic Smart Copolymer for DNA Binding

CQFM—Centro de Química-Física Molecular and IN—Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal
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Received: 6 October 2017 / Revised: 25 October 2017 / Accepted: 2 November 2017 / Published: 4 November 2017
(This article belongs to the Special Issue Polymer Characterization)
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Abstract

A new block copolymer with a temperature-responsive block and a cationic block was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization, with good control of its size and composition. The first block is composed by di(ethylene glycol) methyl ether methacrylate (DEGMA) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA), with the ratio DEGMA/OEGMA being used to choose the volume phase transition temperature of the polymer in water, tunable from ca. 25 to above 90 °C. The second block, of trimethyl-2-methacroyloxyethylammonium chloride (TMEC), is positively charged at physiological pH values and is used for DNA binding. The coacervate complexes between the block copolymer and a model single strand DNA are characterized by fluorescence correlation spectroscopy and fluorescence spectroscopy. The new materials offer good prospects for biomedical application, for example in controlled gene delivery. View Full-Text
Keywords: block copolymer; stimuli responsive polymer; DNA; fluorescence; FCS block copolymer; stimuli responsive polymer; DNA; fluorescence; FCS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ribeiro, T.; Santiago, A.M.; Gaspar Martinho, J.M.; Farinha, J.P. A Cationic Smart Copolymer for DNA Binding. Polymers 2017, 9, 576.

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