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Polymers 2016, 8(10), 339; doi:10.3390/polym8100339

Biphasic Polyurethane/Polylactide Sponges Doped with Nano-Hydroxyapatite (nHAp) Combined with Human Adipose-Derived Mesenchymal Stromal Stem Cells for Regenerative Medicine Applications

1
Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, Wroclaw 51-631, Poland
2
Department of Animal Physiology and Biostructure, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland
3
Electron Microscopy Laboratory, Wroclaw Research Centre EIT+, Wroclaw 54-066, Poland
4
Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow 30-059, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: João F. Mano and Gary L. Bowlin
Received: 5 May 2016 / Revised: 22 August 2016 / Accepted: 31 August 2016 / Published: 11 October 2016
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Abstract

Cartilage and bone tissue injuries are common targets in regenerative medicine. The degeneration of cartilage tissue results in tissue loss with a limited ability to regenerate. However, the application of mesenchymal stem cells in the course of such condition makes it possible to manage this disorder by improving the structure of the remaining tissue and even stimulating its regeneration. Nevertheless, in the case of significant tissue loss, standard local injection of cell suspensions is insufficient, due to the low engraftment of transplanted cells. Introduction of mesenchymal stem cells on the surface of a compatible biomaterial can be a promising tool for inducing the regeneration by both retaining the cells at the desired site and filling the tissue gap. In order to obtain such a cell-biomaterial hybrid, we developed complex, biphasic polymer blend biomaterials composed of various polyurethane (PU)-to-polylactide (PLA) ratios, and doped with different concentrations of nano-hydroxyapatite (nHAp). We have determined the optimal blend composition and nano-hydroxyapatite concentration for adipose mesenchymal stem cells cultured on the biomaterial. We applied biological in vitro techniques, including cell viability assay, determination of oxidative stress factors level, osteogenic and chondrogenic differentiation potentials as well as cell proteomic analysis. We have shown that the optimal composition of biphasic scaffold was 20:80 of PU:PLA with 20% of nHAp for osteogenic differentiation, and 80:20 of PU:PLA with 10% of nHAp for chondrogenic differentiation, which suggest the optimal composition of final biphasic implant for regenerative medicine applications. View Full-Text
Keywords: thermoplastic polyurethane; polylactide; nano-hydroxyapatite; adipose stem cells; biomaterials thermoplastic polyurethane; polylactide; nano-hydroxyapatite; adipose stem cells; biomaterials
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Marycz, K.; Marędziak, M.; Grzesiak, J.; Lis, A.; Śmieszek, A. Biphasic Polyurethane/Polylactide Sponges Doped with Nano-Hydroxyapatite (nHAp) Combined with Human Adipose-Derived Mesenchymal Stromal Stem Cells for Regenerative Medicine Applications. Polymers 2016, 8, 339.

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