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Polymers 2015, 7(11), 2371-2388; doi:10.3390/polym7111519

Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

1
Product Development Lab, Mechanical Engineering Department, Universidad Politecnica de Madrid, c/ Jose Gutierrez Abascal 2, Madrid 28006, Spain
2
Molecular Biology Department, Universidad Autónoma de Madrid, Cantoblanco-Madrid 28049, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Esmaiel Jabbari and Jianxun Ding
Received: 16 July 2015 / Revised: 3 November 2015 / Accepted: 11 November 2015 / Published: 23 November 2015
(This article belongs to the Special Issue Polymers Applied in Tissue Engineering)
View Full-Text   |   Download PDF [3518 KB, uploaded 23 November 2015]   |  

Abstract

This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented. View Full-Text
Keywords: biomedical microdevices; surface topography; fractal geometry; biomimetic devices; cell culture; cell dynamics; tissue engineering biomedical microdevices; surface topography; fractal geometry; biomimetic devices; cell culture; cell dynamics; tissue engineering
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Diaz Lantada, A.; Alarcón Iniesta, H.; García-Ruíz, J.P. Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility. Polymers 2015, 7, 2371-2388.

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