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Polymers 2014, 6(10), 2625-2644; doi:10.3390/polym6102625

Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions

1
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia
2
Polymer Institute, Slovak Academy of Sciences, SK-84541 Bratislava, Slovakia
3
Istituto per lo Studio delle Macromolecole, Consiglio Nazionale delle Ricerche, I-20133 Milano, Italy
4
Institute of Chemistry, Slovak Academy of Science, SK-84538 Bratislava, Slovakia
5
Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Received: 22 July 2014 / Revised: 23 September 2014 / Accepted: 14 October 2014 / Published: 21 October 2014
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Abstract

The bucillamine effect on free-radical-mediated degradation of high-molar-mass hyaluronan (HA) has been elucidated. As HA fragmentation is expected to decrease its dynamic viscosity, rotational viscometry was applied to follow the oxidative HA degradation. Non-isothermal chemiluminometry, thermogravimetry, differential scanning calorimetry, and size-exclusion chromatography (SEC) were applied to characterize resulting HA fragments. Although bucillamine completely inhibited the HA viscosity decrease caused by oxidative system, indicating HA protection from degradation, SEC analysis suggested that some other mechanisms leading to the bucillamine transformations without the decay of the viscosity may come into a play as well. Nonetheless, the link between the reduction of chemiluminescence intensity and disappearance of the differential scanning calorimetry exotherm at 270 °C for fragmented HAs indicates a particular role of the bucillamine in preventing the decrease of HA viscosity. View Full-Text
Keywords: disease-modifying antirheumatic drugs; rotational viscometry; size exclusion chromatography; thermo-analytical methods disease-modifying antirheumatic drugs; rotational viscometry; size exclusion chromatography; thermo-analytical methods
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Baňasová, M.; Valachová, K.; Rychlý, J.; Janigová, I.; Csomorová, K.; Mendichi, R.; Mislovičová, D.; Juránek, I.; Šoltés, L. Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions. Polymers 2014, 6, 2625-2644.

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