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Polymers 2014, 6(10), 2573-2587; doi:10.3390/polym6102573

Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy

1,* , 1,†
,
2,†
,
3,†
and
4,*
1
Zhejiang Pharmaceutical College, Ningbo 315100, Zhejiang, China
2
Department of Medicine, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China
3
Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China
4
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310023, Zhejiang, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 11 August 2014 / Revised: 25 September 2014 / Accepted: 2 October 2014 / Published: 17 October 2014
(This article belongs to the Special Issue Polymers and Polymeric Nanoparticles for Therapy and Imaging)
View Full-Text   |   Download PDF [5479 KB, uploaded 17 October 2014]   |  

Abstract

Mannose polyethylenimine with a molecular weight of 25 k (Man-PEI25k) was synthesized via a phenylisothiocyanate bridge using mannopyranosylphenyl isothiocyanate as a coupling reagent, and characterized by 1H NMR (nuclear magnetic resonance) and FT-IR (Fourier transform infrared spectroscopy) analysis. Spherical nanoparticles were formed with diameters of 80–250 nm when the copolymer was mixed with DNA at various charge ratios of copolymer/DNA (N/P). Gel electrophoresis demonstrated that the DNA had been condensed and retained by the PEI derivates at low N/P ratios. The Man-PEI25k/DNA complexes were less cytotoxic than the PEI complexes with a molecular weight of 25 k (PEI25k) at the same N/P ratio. Laser scan confocal microscopy and flow cytometry confirmed that the Man-PEI25k/DNA complexes gave higher cell uptake efficiency in (Dendritic cells) DC2.4 cells than HeLa cells. The transfection efficiency of Man-PEI25k was higher than that of PEI25k towards DC2.4 cells. These results indicated that Man-PEI25k could be used as a potential DC-targeting non-viral vector for gene therapy. View Full-Text
Keywords: polyethylenimine (PEI); mannose; targeted nanoparticle; gene vector polyethylenimine (PEI); mannose; targeted nanoparticle; gene vector
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hu, Y.; Xu, B.-H.; Xu, J.-J.; Shou, D.; Gao, J.-Q. Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy. Polymers 2014, 6, 2573-2587.

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