Next Article in Journal
Investigation of Second-Harmonic Generation and Molecular Orientation in Electrostatically Self-Assembled Thin Films
Next Article in Special Issue
Activity and Mechanism of Antimicrobial Peptide-Mimetic Amphiphilic Polymethacrylate Derivatives
Previous Article in Journal / Special Issue
Defensins: Potential Effectors in Autoimmune Rheumatic Disorders
Article Menu

Export Article

Open AccessArticle
Polymers 2011, 3(3), 1282-1296; doi:10.3390/polym3031282

Activity and Export of Engineered Nisin-(1-22) Analogs

BiOMaDe Technology Foundation, Nijenborgh 4, 9747 AG Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 4 July 2011 / Revised: 29 July 2011 / Accepted: 11 August 2011 / Published: 12 August 2011
View Full-Text   |   Download PDF [784 KB, uploaded 12 August 2011]   |  

Abstract

The pentacyclic peptide antibiotic nisin, produced by Lactococcus lactis is ubiquitously applied as a food preservative. We previously demonstrated that the truncated nisin-(1-22) has only 10-fold lower activity than nisin. Here we aimed at further developing this tricyclic nisin analog to reach activity comparable to that of nisin. Our data demonstrate that: (1) ring A has a large mutational freedom; (2) the composition of residues 20–22 strongly affects production levels of nisin-(1-22); (3) a positively charged C-terminus of nisin-(1-22) significantly enhances its antimicrobial activity; (4) nisin-(1-22) inhibits in vitro growth of a target strain using different dynamics than nisin.
Keywords: nisin; lantibiotic; peptide; cyclization; Lactococcus nisin; lantibiotic; peptide; cyclization; Lactococcus
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Plat, A.; Kuipers, A.; Lange, J.G.; Moll, G.N.; Rink, R. Activity and Export of Engineered Nisin-(1-22) Analogs. Polymers 2011, 3, 1282-1296.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top