Biocompatibility and Physico-Chemical Properties of Highly Porous PLA/HA Scaffolds for Bone Reconstruction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials of Scaffolds
2.2. Scaffold Preparation Technique
2.3. Characterization of Scaffold
2.3.1. Structure Characterization
2.3.2. Study of Mechanical Properties
2.3.3. Measurement of the Contact Angle
2.4. In Vitro Studies
2.4.1. Cell Culture
2.4.2. Cell Adhesion and Proliferation Analysis
2.5. In Vivo Implantation and Histologic Analysis
2.6. Statistics
3. Results and Discussions
3.1. Microstructure Analysis
3.2. Porosity Calculation
3.3. Sample Composition Analysis
3.4. Mechanical Testing
3.5. FT-IR Analysis
3.6. Assessment of Surface Hydrophilicity
3.7. In Vitro Experiment
3.7.1. Cell Adhesion
3.7.2. Cell Proliferation
3.7.3. Cell Viability
3.8. In Vivo Implantation and Histologic Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Data Availability
References
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Samples | Ultimate Strength, MPa | Young’s Modulus, MPa | Elongation at Break, % |
---|---|---|---|
Cancellous Bone | 80–90 | 50–500 | <1 |
PLA | 1.53 ± 0.22 | 89.01 ± 12.74 | 6.42 ± 2.0 |
PLA/HA 15% | 2.47 ± 0.19 | 177.24 ± 53.62 | 2.23 ± 0.54 |
PLA/HA 20% | 1.41 ± 0.37 | 183.56 ± 39.60 | 1.15 ± 0.55 |
Sample | The Value of the Wetting Angle, ° |
---|---|
PLA | 83.6 ± 1.91 |
PLA/HA 15% | 64.6 ± 1.87 |
PLA/HA 20% | 62.4 ± 4.17 |
PLA | PLA/HA 15% | PLA/HA 20% | |
---|---|---|---|
Polymorphonuclear cells | 2 * | 0 | 1 |
Lymphocytes | 3 | 2 | 1 |
Plasma cells | 1 | 1 | 1 |
Macrophages | 1 | 1 | 1 |
Giant cells | 2 | 1 | 1 |
Necrosis | 0 | 0 | 0 |
Fibrosis | 1 | 1 | 1 |
Fatty infiltrate | 0 | 0 | 0 |
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Zimina, A.; Senatov, F.; Choudhary, R.; Kolesnikov, E.; Anisimova, N.; Kiselevskiy, M.; Orlova, P.; Strukova, N.; Generalova, M.; Manskikh, V.; et al. Biocompatibility and Physico-Chemical Properties of Highly Porous PLA/HA Scaffolds for Bone Reconstruction. Polymers 2020, 12, 2938. https://doi.org/10.3390/polym12122938
Zimina A, Senatov F, Choudhary R, Kolesnikov E, Anisimova N, Kiselevskiy M, Orlova P, Strukova N, Generalova M, Manskikh V, et al. Biocompatibility and Physico-Chemical Properties of Highly Porous PLA/HA Scaffolds for Bone Reconstruction. Polymers. 2020; 12(12):2938. https://doi.org/10.3390/polym12122938
Chicago/Turabian StyleZimina, Anna, Fedor Senatov, Rajan Choudhary, Evgeniy Kolesnikov, Natalya Anisimova, Mikhail Kiselevskiy, Polina Orlova, Natalia Strukova, Mariya Generalova, Vasily Manskikh, and et al. 2020. "Biocompatibility and Physico-Chemical Properties of Highly Porous PLA/HA Scaffolds for Bone Reconstruction" Polymers 12, no. 12: 2938. https://doi.org/10.3390/polym12122938