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Crystals 2017, 7(8), 242; doi:10.3390/cryst7080242

A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software

1
Signalling and Transcription Laboratory, Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK
2
Present address: Division of Cancer Studies, Faculty of Life Sciences & Medicine, Guy’s Campus, King’s College London, London SE1 1UL, UK
3
Research Complex at Harwell, Rutherford Appleton Laboratory, Oxford OX11 0FA, UK
4
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
5
Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
*
Author to whom correspondence should be addressed.
Received: 11 July 2017 / Revised: 26 July 2017 / Accepted: 31 July 2017 / Published: 4 August 2017
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Abstract

The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred X-ray diffraction datasets obtained from 50 crystals of a complex formed between the SRF transcription factor, its cognate DNA, and a peptide from the SRF cofactor MRTF-A. This structure is currently in the process of being fully solved. While full details of the structure are not yet available, the repeated application of BLEND on data from this structure, as they have become available, has made it possible to produce electron density maps clear enough to visualise the potential location of MRTF sequences. View Full-Text
Keywords: BLEND; multiple crystals; hierarchical cluster analysis; protein-protein-DNA complex BLEND; multiple crystals; hierarchical cluster analysis; protein-protein-DNA complex
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mylona, A.; Carr, S.; Aller, P.; Moraes, I.; Treisman, R.; Evans, G.; Foadi, J. A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software. Crystals 2017, 7, 242.

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