Next Article in Journal
Statistically Guided Synthesis of MoV-Based Mixed-Oxide Catalysts for Ethane Partial Oxidation
Previous Article in Journal
Metal–Organic Frameworks-Based Catalysts for Biomass Processing
Article Menu

Export Article

Open AccessArticle
Catalysts 2018, 8(9), 369; https://doi.org/10.3390/catal8090369

Efficient Physisorption of Candida Antarctica Lipase B on Polypropylene Beads and Application for Polyester Synthesis

1
Department of Agrobiotechnology, Institute of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Strasse 20, 3430 Tulln an der Donau, Austria simone.weinberger@boku.ac.at (S.W.)
2
Department of Chemistry, Green Chemistry Centre of Excellence, University of York, Heslington, York YO10 5DD, UK
3
Division Enzymes & Polymers, Austrian Centre of Industrial Biotechnology, Konrad Lorenz Strasse 20, 3430 Tulln an der Donau, Austria
*
Author to whom correspondence should be addressed.
Received: 27 July 2018 / Revised: 29 August 2018 / Accepted: 31 August 2018 / Published: 31 August 2018
(This article belongs to the Special Issue Biocatalytic Polymer Synthesis)
Full-Text   |   PDF [1364 KB, uploaded 3 September 2018]   |  

Abstract

In the present work, Candida antarctica lipase B (CaLB) was adsorbed onto polypropylene beads using different reaction conditions, in order to investigate their influence on the immobilization process and the enzyme activity of the preparations in polymerization reactions. In general, lower salt concentrations were more favorable for the binding of enzyme to the carrier. Polymerisation of dimethyl adipate (DMA) and 1,4-butanediol (BDO) was investigated in thin-film systems at 70 °C and at both atmosphere pressure (1000 mbar) and 70 mbar. Conversion rates and molecular masses of the reaction products were compared with reactions catalyzed by CaLB in its commercially available form, known as Novozym 435 (CaLB immobilized on macroporous acrylic resin). The best results according to molecular weight and monomer conversion after 24 h reaction time were obtained with CaLB immobilized in 0.1 M Na2HPO4\NaH2PO4 buffer at pH 8, producing polyesters with 4 kDa at conversion rates of 96% under low pressure conditions. The stability of this preparation was studied in a simulated continuous polymerization process at 70 °C, 70 mbar for 4 h reaction time. The data of this continuous polymerizations show that the preparation produces lower molecular weights at lower conversion rates, but is comparable to the commercial enzyme concerning stability for 10 cycles. However, after 24 h reaction time, using our optimum preparation, higher molecular weight polyesters (4 kDa versus 3.1 kDa) were obtained when compared to Novozym 435. View Full-Text
Keywords: enzyme immobilization; polyester synthesis; Candida antarctica lipase B; green synthesis; protein adsorption; biobased plastics enzyme immobilization; polyester synthesis; Candida antarctica lipase B; green synthesis; protein adsorption; biobased plastics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Weinberger, S.; Pellis, A.; Comerford, J.W.; Farmer, T.J.; Guebitz, G.M. Efficient Physisorption of Candida Antarctica Lipase B on Polypropylene Beads and Application for Polyester Synthesis. Catalysts 2018, 8, 369.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Catalysts EISSN 2073-4344 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top