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Catalysts 2018, 8(3), 117; https://doi.org/10.3390/catal8030117

Functional Analysis of Methylomonas sp. DH-1 Genome as a Promising Biocatalyst for Bioconversion of Methane to Valuable Chemicals

1
Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 17104, Korea
2
Climate Change Research Division, Korea Institute of Energy Research, Daejeon 34129, Korea
3
Department of Biosience and Biotechnology, Konkuk University, Seoul 05029, Korea
4
Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
*
Authors to whom correspondence should be addressed.
Received: 27 January 2018 / Revised: 7 March 2018 / Accepted: 15 March 2018 / Published: 16 March 2018
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Methylomonas sp. DH-1, newly isolated from the activated sludge of a brewery plant, has been used as a promising biocatalytic platform for the conversion of methane to value-added chemicals. Methylomonas sp. DH-1 can efficiently convert methane and propane into methanol and acetone with a specific productivity of 4.31 and 0.14 mmol/g cell/h, the highest values ever reported, respectively. Here, we present the complete genome sequence of Methylomonas sp. DH-1 which consists of a 4.86 Mb chromosome and a 278 kb plasmid. The existence of a set of genes related to one-carbon metabolism and various secondary metabolite biosynthetic pathways including carotenoid pathways were identified. Interestingly, Methylomonas sp. DH-1 possesses not only the genes of the ribulose monophosphate cycle for type I methanotrophs but also the genes of the serine cycle for type II. Methylomonas sp. DH-1 accumulated 80 mM succinate from methane under aerobic conditions, because DH-1 has 2-oxoglutarate dehydrogenase activity and the ability to operate the full TCA cycle. Availability of the complete genome sequence of Methylomonas sp. DH-1 enables further investigations on the metabolic engineering of this strain for the production of value-added chemicals from methane. View Full-Text
Keywords: complete genome sequence; methane; Methylomonas sp. DH-1; secondary metabolites complete genome sequence; methane; Methylomonas sp. DH-1; secondary metabolites
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Nguyen, A.D.; Hwang, I.Y.; Lee, O.K.; Hur, D.H.; Jeon, Y.C.; Hadiyati, S.; Kim, M.-S.; Yoon, S.H.; Jeong, H.; Lee, E.Y. Functional Analysis of Methylomonas sp. DH-1 Genome as a Promising Biocatalyst for Bioconversion of Methane to Valuable Chemicals. Catalysts 2018, 8, 117.

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