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Catalysts 2017, 7(9), 271; doi:10.3390/catal7090271

Catalytic Characteristics of New Antibacterials Based on Hexahistidine-Containing Organophosphorus Hydrolase

Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
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Received: 30 August 2017 / Revised: 8 September 2017 / Accepted: 11 September 2017 / Published: 14 September 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Catalytic characteristics of hexahistidine-containing organophosphorus hydrolase (His6-OPH) and its enzyme-polyelectrolyte complexes with poly-l-glutamic acid or poly-l-aspartic acid (His6-OPH/PLD50), hydrolyzing organophosphorous compounds, and N-acyl homoserine lactones were studied in the presence of various antibiotics (ampicillin, gentamicin, kanamycin, and rifampicin). The antibiotics at concentrations below 1 g·L−1 had a negligible inhibiting effect on the His6-OPH activity. Mixed inhibition of His6-OPH was established for higher antibiotic concentrations, and rifampicin was the most potent inhibitor. Stabilization of the His6-OPH activity was observed in the presence of antibiotics at a concentration of 0.2 g·L−1 during exposure at 25–41 °C. Molecular docking of antibiotics to the surface of His6-OPH dimer revealed the antibiotics binding both to the area near active centers of the enzyme subunits and to the region of contact between subunits of the dimer. Such interactions between antibiotics and His6-OPH were verified with Fourier-transform infrared (FTIR) spectroscopy. Considering all the results of the study, the combination of His6-OPH/PLD50 with β-lactam antibiotic ampicillin was established as the optimal one in terms of exhibition and persistence of maximal lactonase activity of the enzyme. View Full-Text
Keywords: hexahistidine-containing organophosphorus hydrolase; antibiotic; ampicillin; gentamicin; kanamycin; rifampicin; inhibition; enzyme stability hexahistidine-containing organophosphorus hydrolase; antibiotic; ampicillin; gentamicin; kanamycin; rifampicin; inhibition; enzyme stability
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MDPI and ACS Style

Maslova, O.; Aslanli, A.; Stepanov, N.; Lyagin, I.; Efremenko, E. Catalytic Characteristics of New Antibacterials Based on Hexahistidine-Containing Organophosphorus Hydrolase. Catalysts 2017, 7, 271.

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